FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350400
Sequences flagged as poor quality0
Sequence length301
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG242446.918949771689498No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT161994.6230022831050235No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA141844.0479452054794525No Hit
AAAACCCCACTCTATCTTACAACCTAAATATCCCCTGTAGAACCATGTCG129463.694634703196347No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA128223.659246575342466No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA124523.5536529680365296No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG119763.417808219178082No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT101752.903824200913242No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC98682.8162100456621No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC95162.7157534246575343No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA86582.470890410958904No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT86172.459189497716895No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC83732.389554794520548No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC72032.055650684931507No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC70582.014269406392694No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA69451.9820205479452055No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC56531.6132990867579906No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA55821.5930365296803652No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT52421.4960045662100456No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA48381.3807077625570776No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT35851.0231164383561644No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA22570.64412100456621No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC22160.632420091324201No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA18670.5328196347031964No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT16330.46603881278538817No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT13530.3861301369863014No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC13510.3855593607305936No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACCCCTAAAACCTCAACAAA12500.3567351598173516No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC11320.3230593607305936No Hit
TAAAACTAAACCCCACATACCTTCCCCAAAAAAACCTACTCATAAACTGT9430.26912100456621No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC9180.261986301369863No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA8310.23715753424657535No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC8020.22888127853881277No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA7050.2011986301369863No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC5550.1583904109589041No Hit
TAATCTAAACCTCCTAACCTCAAACAATTTACCCACCTTAACCTCCCAAA5400.1541095890410959No Hit
TAATCTAAACCTCCTAACCTCAAACCATAAAAAAATCATCTACCCTCATG4390.1252853881278539No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAACCCAATTCACCCAT4210.12014840182648402No Hit
ATAATACTCCTTTATCCCAATCCCCAAGACCCTTCACCAAAAATACTAAA4200.11986301369863014No Hit
AAACCTATCAAAACCTCCCCACAAATCTCTATCTTCCACCCTAATGTAGA4170.11900684931506848No Hit
CCTAAAAAAACAACACTCCAAACACTCCAAACCCCACTCTATCTTACAAC4060.1158675799086758No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT3960.113013698630137No Hit
CCTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA3530.1007420091324201No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA3530.1007420091324201No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)