FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325952
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG159464.892131356764186No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT134544.127601610053014No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT106863.2783968191635577No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA87632.6884326526605147No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA83472.5608064991164343No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA81572.5025157078342826No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA71952.207380227763597No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT69532.133136167288435No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC68352.0969345179658356No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC62601.9205281759277442No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT61161.8763498920086392No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC60921.8689868446887887No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC60261.8487384645591989No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC49581.5210828588258394No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA47721.4640192420969957No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC42091.2912944237188297No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG41971.2876129000589045No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC40151.2317764578833692No Hit
TAAAACTAAACCCCACATACCTTCCCCAACCCAAACACAACACTAACACA38741.188518554879246No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA38351.1765536029844885No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT38151.1704177302179462No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA36211.1108997643824856No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA35571.0912649715295504No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA32841.0075103082662478No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA32040.9829668172000785No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA31040.952287453367367No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA30430.9335730414294129No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT29470.9041208521500098No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC28450.872827901040644No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA27960.8577950127626154No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA26500.8130031415668565No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA22440.6884449244060475No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACTAAAAAATAAACCCCTA21590.6623674651482426No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC19320.5927253092479874No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT18500.567568230905164No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA17340.5319801688592186No Hit
AAAAAAATCCTTCCAACCAATAAAACTAACAATACCTACCCAACATGTAG16480.5055959159630866No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC15830.4856543294718241No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC13310.4083423326133909No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA12760.3914686825053995No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC12180.37367465148242685No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT11630.3568010013744355No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA11450.35127871588454745No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAGCCCCCACCACCCGCAA11030.3383933830748086No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCACCT10970.33655262124484586No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA10900.33440506577655604No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACGCCAAACCCCCACAAAAC10520.32274690752012564No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA10070.30894119379540547No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACGAACATTAAAACCTTCATC9920.30433928922049874No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA9650.2960558609856666No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC9200.2822501472609464No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC8910.27335313174946No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA8810.2702851953661889No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA7820.23991262517180445No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA7600.2331631651286079No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC7220.2215050068721775No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAACTCACCCA7210.2211982132338504No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA7070.21690310229727075No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT7000.21475554682898096No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC6820.2092332613390929No Hit
AAAAATCCTAAAAACCCCAAAACACAAATTCCAAAATCTACCTAATGTAG6290.19297319850775574No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACACTA6060.18591694482623208No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA5940.1822354211663067No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC5920.18162183388965247No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT5890.1807014529746711No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT5870.1800878656980169No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCGATCCACTC5670.17395199293147456No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCAATCCACTC5380.16505497741998823No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC5380.16505497741998823No Hit
AAACTACAAAATCAAAACCAACCCAAAACCGCACCAAACCCCCACAAAAC5200.15953269193010014No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA4810.14756774003534262No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT4750.14572697820537994No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC4500.13805713724720203No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTCCTAACACC4400.13498920086393087No Hit
ATACAAAACAAACCCCTATCTCCTCTGCCGCGACCTTAAAAAACAATACC4270.13100088356567838No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCGATCCACTC4250.13038729628902415No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC4100.12578539171411743No Hit
AAAAAAATCCTTCCAACCAACAAAACTAACAATACCTACCCAACATGTAG3920.12026310622422934No Hit
TAAAACTAAACCCCACATACCTTCCAACATAATAATTAAAACTAAAAAAC3860.11842234439426663No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCACTCTAACCACCCTCG3670.11259326526605144No Hit
CCCCTAATAAAAACTTCCTTCCACTAAACAAAAATCAAACCCAAAATTCT3600.11044570979776164No Hit
CAACCATAAATACACAAAAAACAACCCATACCACTACTAAATACACGACC3550.10891174160612607No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA3540.10860494796779893No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)