FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365831
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG180254.927138487443656No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT162534.442761821715491No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT117043.199291476118754No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT107462.93742192433118No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA86872.3745937331718743No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA83112.271814034349194No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA80992.213863778630023No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC70881.9375066629126563No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC69341.8954107224374097No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA69151.8902170674437102No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC66591.8202394001601834No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA63011.7223800060683758No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA62081.696958431625532No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA58881.6094863475211232No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA56241.537321878134986No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT52411.4326287274725216No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA50341.3760452230674818No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA48301.3202817694509212No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC45151.2341764366606438No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC43911.2002810040701855No Hit
AAAACCCCACTCTATCTTACAACCTAAATATCCCCTGTAGAACCATGTCG37561.026703587175499No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG37111.0144028253483166No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA37051.0127627237713588No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA36671.0023754137839604No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC34320.9381381020197852No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT33160.9064294715319369No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT32920.8998690652241061No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC32710.8941287097047544No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA32170.8793677955121353No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA29930.8181373366390491No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC29430.8044698234977353No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA27730.7580002788172681No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA27340.7473396185670432No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCACACCACCCACAA25250.6902094136363511No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC23790.6503002752637147No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC18970.5185454485814488No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA18660.5100715904338342No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA18660.5100715904338342No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC17730.4846500159909904No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC17620.4816431630999013No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC16610.4540347865544473No Hit
TAAAACTAAACCCCACATACCTTCCCCAACCCAAACACAACACTAACACA15800.4318934152655188No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA14780.4040116884572385No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC14440.394717779521145No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA14220.38870407373896687No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT13820.3777700632259158No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT13720.375036560597653No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA13370.3654693013987333No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC13040.3564487427254661No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC12190.33321397038523254No Hit
TAATCTAAACCTCCTAACCTCAAACGATCCATCTACTTCTACCTCCGCCT11860.32419341171196536No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT11430.3124393504104354No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA9940.27171016124932No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC9910.2708901104608412No Hit
AAAACCCCACTCTATCTTACAACCTAAATATCCCCTGTAGAACCATATCG9750.2665165062556208No Hit
AACCTCCTCTCCAAAACTAAATCTTACACGCCCCCAAAATTAATTTATAC9730.2659698057299682No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT9690.2648764046786631No Hit
CAAAACAAACAACACTAAAACCAAATAACCTTCTCAAAAAACCCTTTCTG9050.24738198785778132No Hit
ATAATACTCCTTTATCCCAATCCCCAAAGCCCTTCACCAAAAATACTAAA8970.2451951857551711No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA8490.2320743731395098No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACA7560.20665279869666592No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCACTTACCAATCCACTC7490.20473934685688203No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCAATCCACTC7020.19189188450404696No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC6970.19052513318991557No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAGGGCGACCATCAAACA6750.18451142740773743No Hit
CAACTACCAAACCCTAAAAAACCACACACACACAAACCATATCCAAACCA6440.17603756926012284No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT6440.17603756926012284No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC5740.1569030508622834No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCGCC5330.145695690086406No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC5110.1396819843042279No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACACTAAAACCTTCATC4780.13066142563096075No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC4650.12710787221421913No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTACAACTCTAAA4510.12328096853465124No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC3860.10551320145094319No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC3810.1041464501368118No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC3710.10141294750854904No Hit
CCTAAAAAAACAACACTCCAAACACATACACCCAAACCCTAAATCCCACC3660.10004619619441765No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)