FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339653
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAGGGTGGAAGATAGAGATTTGTGTTTATAGGGTTTAGGAAGGCGGGTA165814.881746959396796No Hit
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTCGGAAGTATGGTATTT121503.577180239833006No Hit
GTTGGGGATTGAGGTTTTTAGAGTAGAGGAGTTAGTTGTGGGTATTAGTG98992.9144450365520105No Hit
GATTGTTTAGGGAGGGGGTTAATAGCGTGTTAGTATTGAGGTTAGGTTTG88512.6058948397334927No Hit
TGATTTTGTGAGTTTATAGGTTTAGGTTTTAGTAGTAGAATTATTTTGGT85092.505203840390045No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTTGTTTGAGGTTTTTTGT81072.386847753442484No Hit
GTGAAGTTTTTGGTTTTTTGTTGTATAGATGAGGTTGGGATGTAAGGAGA78602.314126476138883No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTTGTGTAGAGGGTTCGTTGGGGTTT77862.292339534760476No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTTCGTTTATC74912.2054861873735843No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTCGTTTGAGGTTTTTTGT67972.0011600074193368No Hit
GGTGGGGAATAAGGTTTTGTATTTATTGAGTTTTTGTTATAGGTTTGGGT65771.9363880195375869No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGCGTCGGGTA62471.8392300377149622No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGT57661.697614918755318No Hit
TGGTTTTTATTTTTGTGTTTTTTGGGGATGGTTTTTAAGGATAGTATGTT56521.664051252307502No Hit
GTTAAAAGAATTTGGAAGGGTTTGTGAGATATTGGATTGCGGGGTAATAA51361.512131498912125No Hit
GGTTGTAGGAGGTTTTTAGTAAGGGAGGGGTTGTAGGAGTGTTTTTGGGG51261.5091873176447728No Hit
AGAAGGGTTAGTTATAGGGGAGGATGTGGTATAGGTGTTTATGGGATTTT50611.4900501394069832No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTT48781.4361716222144365No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGTGGTGTTGAT45781.3478461841938685No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTCGGTGATTGTTTCGGAGGAGAGG44021.2960285938884686No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGTGGGGGGTAT42681.2565765649059482No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTTCGTTATTTTACGATGAGTTAG39631.1667790362517039No Hit
GAATTGAAGGAGAATTTATTTGTGGTTGTTATCGTTTTATTAGGATAGTT38191.1243828260018314No Hit
TGGGATATTTTTGATTTATATTTGGGTTTTAGGGAGTTAAGGTATTGTGG32810.9659858738182793No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTTATGGGATAGGGAGTAT32740.9639249469311326No Hit
GGAAAGTTAGGTAGTGAGTTGTTTGATATTTGTAGTATTTATGTTGGAGT30360.8938534327681485No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTAGTATTTTTGATTTTTTTGATT30240.890320415247326No Hit
TGTTGGGTAGGTATTGTTAGTTTTGTTGTATGGATGAAGGCGTGAAGGTT29930.8811934533185338No Hit
GTTGGTTTTTTTGGGGTGAAGCGGTTTTTATTTTCGTTGATGAACGGTTT29050.8552846581658339No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTTAGTTTTATTTTTAAATT28190.8299646992666044No Hit
GATAGGTTTGGAGATAGTTTTGTTTAAAGATATTTAGTATTTTAGTGGTA24260.7142583754596603No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGTGTTATTTTGTGATGGGTTAG24090.7092532673051615No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTCGTGTAGAGGGTTCGTTGGGGTTT21250.625638519312357No Hit
GGAGTTGAGTGGTATAGTTGGTATTATTACGTATTAGTAGAGGTTTTTTG20900.6153338848766241No Hit
GGAAAGTTAGGTAGTGAGTTGTTTGATATTTGTAGTATTTACGTTGGAGT19930.5867753265833071No Hit
GGGGTGTATTTATTAGTTTTAGGGATGTATTTTTAGGTTTCGGGGGTTTA19540.5752930196406332No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGTGTGAAGAAGTGG19390.5708767477396048No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTGTTT18330.5396684263056708No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGCGGGGGGTAT17000.5005108154498856No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGCGGTGTTGAT15860.46694714900206974No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTG15250.4489876432712209No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTTGGGGTAAGTGTTTATTTTTTTT15180.4469267163840743No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTAATTAATTGAGAAGTTGT14360.4227844299917858No Hit
AATTTGTTGTGGGTGATTATGGTGTATGGAGTTAGTGTGGATTTGTGTGG13800.40629701489461306No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTAATTAATTGAGAAGTTGTT13340.39275378106479253No Hit
TTGTGTGATGGGGATAGTTTATTTTTTATTTTTTGAGTTTTTATATTTAC13180.388043091037029No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTCGTGTAGAGCGTTCGTTGGGGTTT13130.38657100040335285No Hit
GGGGATATTTAGGTTTGTTTTTTTTTGAGATTTAAAGGGGGTTGGTATTT12590.3706724215596506No Hit
GAATTGAAGGAGAATTTATTTGTGGTTGTTATTGTTTTATTAGGATAGTT10630.31296646871954614No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTCGGGGTGTAGGTGGCGTTGAT9690.2852911648064348No Hit
GATAGGTTTGGAGATAGTTTTGTTTAAAGATATTTAGCATTTTAGTGGTA9540.2808748929054064No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGTTT9440.27793071163805416No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGCGGGGGGTGT9370.27586978475090757No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTTTT9340.27498653037070186No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGCGTGAAGAAGTGG9150.2693925859627326No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTATGGGATAGGGAGTATT9120.2685093315825269No Hit
TTTTTAGGTGAGATTAGGGATTTGGGCGTGGGGGGCGGTTAAAGGTATTT8540.2514330802318837No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTTGTGGTGGGGGGTAT8360.24613355395064962No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTAATTAATTGAGAAGTTGTTT8030.23641775576838717No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTT7900.2325903201208292No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGCGGGGGGTAT7800.2296461388534769No Hit
GTTGGTTTTTTTGGGGTGAAGCGGTTTTTATTTTCGTTGATGAATGGTTT7780.22905730260000648No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTTCGTTTATT7290.21463081438998036No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGCAGGTGGCGTTGAT7270.2140419781365099No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTTGTGGCGGGGGGTAT7190.2116866331226281No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTCGTTTATCG7170.21109779686915767No Hit
TTTAAGGTTTGTGTTGATGTTAATGTTATAGGTTACGATAAGGTATGTTA6780.19961548992648379No Hit
GTTAAAAGAATTTGGAAGGGTTTGTGAGATATTGGATTGTGGGGTAATAA6650.19578805427892584No Hit
GATTGTTTAGGGAGGGGGTTAATAGTGTGTTAGTATTGAGGTTAGGTTTG6540.19254945488483835No Hit
GATAGGTTTGGAGATAGTTTTGTTTAAAGATATTTAGTATTTCAGTGGCA6410.1887220192372804No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGTGGCGTTGAT5910.17400111290051906No Hit
GTTGGTTTTTTTGGGGTGAAGCGGTTTTTATTTTCGTTGATGAACGGTCT5620.16546298722519748No Hit
GGTTGTAGGAGGTTTTTAGTAAGGGAGGGGTTGTGGGAGTGTTTTTGGGG5290.155747189042935No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTGTTTTT5240.15427509840925885No Hit
TTTGGAGAGGAGGTTTTTAGATTTTATTTTGGTGGTATAGTGTTTTTTTT5130.15103649901517135No Hit
AGGTATTAGGGAGTTGTATGGGGTTGGTTTTGATTTTGTAGTTTAGACCA5080.14956440838149523No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTATTTTGTGATGGGTTAG5020.14779789962108386No Hit
TGGGTTGTGATTAGATGGTAGAAAGGGGTTTTAGTTAGGTTGATAATGGT4950.14573697273393726No Hit
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTCGGAAGTATGGCATTT4850.142792791466585No Hit
TTGTGTGATGGGGATAGTTTATTTTTTATTTTTTGAGTTTTTATATTTAT4840.1424983733398498No Hit
GATAGGTTTGGAGATAGTTTTGTTTTTGTGGTTTTTTAGGGTTTGGTAGT4820.14190953708637935No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGCGTTGGGTA4720.13896535581902708No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTTAATTAATTGAGAAGTTG4690.13808210143882138No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGACGTTGAGCGTGAAGAAGTGG4640.13661001080514523No Hit
TAGGTTTTATTGTGAGGATGATTTGGGTGATATGTTGTTGGTTGTTTTGA4350.12807188512982368No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGTGTTATTTTGTGATGGGTTAG4240.12483328573573618No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTAGTTTTATTTTTAAATTT4210.1239500313555305No Hit
GGGGATATTTAGGTTTGTTTTTTTTTGGGTTTGGTGTTTTTGGAGTTTTG4170.12277235884858959No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTCGTGTGGAGGGTTCGTTGGGGTTT4150.12218352259511915No Hit
TAGGTTTTATTGTGAGGATGATTTGGGTGATACGTTGTTGGTTGTTTTGA3830.11276214253959187No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTGTTTT3810.11217330628612143No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGCGCAGGCGGTGTTGAT3760.1107012156524453No Hit
TTTAAGGTTTGTGTTGATGTTAATGTTATAGGTTATGATAAGGTATGTTA3700.10893470689203394No Hit
GGGGATATTTAGGTTTGTTTTTTTTGAGATTTAAAGGGGGTTGGTATTTG3620.10657936187815212No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)