FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000888430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451898
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTCGTTTGAGGTTTTTTGT365138.079920690067228No Hit
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTCGGAAGTATGGTATTT228875.064638480365038No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTCGAATTTTTTTTTTTCGT204964.535536780423901No Hit
GTGAAGTTTTTGGTTTTTTGTTGTATAGACGAGGTTGGGATGTAAGGAGA172303.8128073149250494No Hit
GTTGGGGATTGAGGTTTTTAGAGTAGAGGAGTTAGTTGTGGGTATTAGTG163113.609442838870719No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTTCGTTATTTTACGATGAGTTAG158003.496364223785013No Hit
TTAGGGTGGAAGATAGAGATTTGTGTTTATAGGGTTTAGGAAGGCGGGTA157463.484414624539166No Hit
GGGAGATTAAGTAGGTGAGTGGTTTGATGATATAGGGGTTAAAGGTATAT147363.2609128608668323No Hit
TGGGATATTTTTGATTTATATTTGGGTTTTAGGGAGTTAAGGTATTGCGG140443.107780959420046No Hit
GATTGTTTAGGGAGGGGGTTAATATTTTCGTAGTTTGTGAATTTGTTTTA138013.054007762813732No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTCGAATTTTTTTTTTCGTT130992.8986629726177147No Hit
GTTAAAAGAATTTGGAAGGGTTTGTGAGATATTGGATTGCGGGGTAATAA130372.8849430623724825No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTAGTATTTTCGATTTTTTTGATT121802.6952984965633835No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGCGTCGGGTA118132.6140854794666053No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTCGGTGATTGTTTCGGAGGAGAGG69891.5465879468375607No Hit
TGTTGGGTAGGTATTGTTAGTTTTGTTGTATGGATGAAGGCGTGAAGGTT49661.0989205528681252No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTCGAATTTTTTTTTTTTCG44870.9929231817799592No Hit
TTGTGTGATGGGGATAGTTTATTTTTTATTTTTTGAGTTTTTATATTTAC44410.9827438935334964No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTCGGTGATTGTTTCGTAGGAGAGG38390.849527990829789No Hit
GTTGGGGATTGAGGTTTTTAGAGTAGAGGAGTTGGTTGTGGGTATTAGTG33320.7373345312437762No Hit
TGAGGGTAGATGATTTTTTTATGGTTTGAGGTTAGGAGGTTTAGATTAAG32800.725827509747775No Hit
GGAGTTGAGTGGTATAGTTGGTATTGAGTGTTTGTAGTTTTTTTAGGTTT31440.6957322227582331No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTTCGTCATTTTACGATGAGTTAG30480.6744884907656152No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTCGAATTTTTTTTTCGTTT29630.6556789363971516No Hit
GAATTTTGGGTTTGATTTTTGTTTTTTTTTATGAGTTTTGAATGTTTAGA28810.6375332486534572No Hit
TTAGGGTGGAAGATAGAGATTTGTGTCTATAGGGTTTAGGAAGGCGGGTA26280.5815471632979125No Hit
TTTAAGGTTTGTGTTGATGTTAATGTTATAGGTTACGATAAGGTATGTTA23170.5127263231968276No Hit
GTTTATGAGTAGGTTTTTTTGGGGTTTAGTTTTAAGACCAAGTCTCTGCT22610.5003341462011338No Hit
TAGGTTTTATTGTGAGGATGATTTGGGTGATACGTTGTTGGTTGTTTTGA22190.49104001345436366No Hit
GGGTTTAGGTTGTTGAGTTTTGGTCGGGAGTTTTGTGTGGGTATAGTTTG21550.4768775254592851No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTATTTCGCGATGGGTTAG21370.4728943257106692No Hit
AGGTATTAGGGAGTTGTATGGGGTTGGTTTTGATTTTGTAGTTTAGACCA19290.426866239726664No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTTTTGAATTTAGTAGTTTTGATTGG16770.3711014432460422No Hit
GATAGGTTTGGAGATAGTTTTGTTTTTGTGGTTTTGTATGGTATAGTTTT16720.36999499887142673No Hit
GATAGGTTTGGAGATAGTTTTGTTTTTGTGGTTTTTTAGGGTTTGGTAGT15580.3447680671301931No Hit
TTAGGGTGGAAGATAGAGATTTGTGGGGAGGTTTTGATAGGTTTAGACCA14710.3255159350118832No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCTCGTTATTTTACGATGAGTTAG13130.2905522927740331No Hit
TTTGGAGAGGAGGTTTTTAGATTTTATTTTGGCGGTATAGCGTTTTTTTT12870.2847987820260324No Hit
TGTTGGGTAGGTATTGTTAGTTTTATTGGTTGGAAGGATTTTTTTAGACC11720.25935056140987567No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTCGAATTTTTTTTTTTTGT11410.2524906062872595No Hit
TGGGTTGTGATTAGATGGTAGAAAGGGGTTTTAGTTAGGTTGATAATGGT9160.20270060942956153No Hit
GATAGGTTTGGAGATAGTTTTGTTTTGGTGGTTTTTTAGGGTTTGGTAGT7630.1688434115663269No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTCGAATTTTTTTTTTTTTC7520.1664092339421728No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTGGTTTTAGTGTTGTTTGTTTTGAG7450.16486021181771107No Hit
AGGAAGGTAGTTAAGAGGGGTATTTAGGTTGTTTTTTATTTTTTGTAATT7190.15910670106971042No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTCGTTTGAGGTTTTTGTA6860.15180416819724807No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTCGAATTTTTTTTTTTGTT5890.13033914732970717No Hit
GTTGGTTTTTTTGGGGTGAAGTTGGTATATGTTTTTAAAGGATAGTGAAA5540.12259403670739857No Hit
GAATTTTGGGTTTGATTTTTGTTTTTTTTATGAGTTTTGAATGTTTAGAG5430.12015985908324445No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTCGAATTTTTTTTTTTTTG5050.11175088183616656No Hit
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTCGGAAGTATGGTATTC4620.10223546021447318No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)