FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000888431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451898
Sequences flagged as poor quality0
Sequence length301
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCGAACCCAACTCGTTCCGCGCCAACGT434729.619869970657096No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA346847.675183337832873No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT216594.792895741959469No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA185454.103802185448928No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG171683.799087404679817No Hit
TACTATCCACACTCTCTTACCTCCCGACCCCCGCACTCCCTTCTAACACC170113.76434505131689No Hit
CCCAAAAATTAATAAATAAACCCCACAATCATCAACTAAACTAAAATCCC141573.1327866022863566No Hit
AAAACAAAAAAAACTCCTAATCCCTCCTACGAATTTCTATATTCAAATAT132982.942699458727412No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCGATCCACTC131592.911940305113101No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA123132.7247299169281565No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC122732.715878361931232No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT117842.607668102093835No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA112102.4806482878879748No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG110242.439488557152278No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA49451.0942734864947399No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCCTACCCAAATACTCTTCTCAA40640.899317987687487No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC39890.8827213220682544No Hit
TAAACACCAAACCCAAAAAAATAAATCCACCTACAACTTACACTATAAAC32620.7218443099991592No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA31680.7010431557563875No Hit
TAATCTAAACCTCCTAACCTCAAACCATAAAAAAATCATCTACCCTCATG30870.6831187568876163No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATGAATAACT22180.49081872457944054No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA21710.4804181474580547No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC20530.45430606021712866No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT19960.4416925943465118No Hit
TAAAACTAAACCCCAAAAAAACCTACTCATAAACTGTAGAACCATGTCGT19910.44058614997189627No Hit
AAACCTATCAAAACCTCCCCACAAATCTCTATCTTCCACCCTAATGTAGA17630.39013228648942905No Hit
TAAAACTAAACCCCACATACCTTCCCTTCCTTTCCCATATCCAAAACTAC17510.3874768199903518No Hit
CAACTACCAAACCCTAAAAAACCACAAACACTCAACACCAACCCATAAAA16860.3730930431203502No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA16200.3584879773754254No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC15130.3348100677586535No Hit
AAAACCCCACTCTATCTTACAACCTAAATATCCCCTGTAGAACCATGTCG14660.3244094906372677No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT14580.32263917963788286No Hit
CCTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA14130.3126811802663433No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATGTCTCTCTAAAAATTTCTAAA13810.30559993626880405No Hit
ATAATACTCCTTTATCCCAATCCCCACTCCAACTTAAACCCCCCAATCCC13580.3005102921455727No Hit
AAAAAAATCCTTCCAACCAATAAAACTAACAATACCTACCCAACATGTAG12530.27727496027864695No Hit
CCCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA9330.20646252030325427No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAGCGCACACTTCAAT9060.20048772068033052No Hit
CAACTACCAAACCCTAAAAAACCACCAAAACAAAACTATCTCCAAACCTA7880.1743756334394045No Hit
CAAAACAAACAACACTAAAACCAAATAACCTTCTCAAAAAACCCTTTCTG7800.17260532244001967No Hit
ACTCTAAAAAACCAACTCCTCCAAAACACACCACCTATTAAAACACCAAC7000.15490221244617147No Hit
AAACACAAACCTATCTCTTATCCCCTACGCCCGAAAAAAAAAACGACACA5900.13056043620463026No Hit
CCCAAAAATTAATAAATAAACCCCACAATCACCAACTAAACTAAAATCCC5570.12325790333216789No Hit
AATACCTTAACTCCCTAAAACCCAAGTATAAATCAAAAATATCCCAAAAC5440.12038114795816755No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA5050.11175088183616656No Hit
CCCAAAAATTAATAAATAAACCCCAATCATCAACTAAACTAAAATCCCTA4840.10710381546278143No Hit
AACCCCAAATACAAAAAAATACACCATAATAAAAAAAAATAAAAAATATA4520.10002257146524217No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)