FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294007
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG121784.142078249837589No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT119404.06112779627696No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT97413.3131864207314794No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT84892.8873462196478314No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA78882.6829293180094353No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA72732.4737506249851196No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC64742.2019883880315776No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA61042.0761410442608508No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC59512.024101466971875No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC58361.9849867520161084No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC56541.9230834639991565No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA56331.9159407769202774No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA52361.7809099783338493No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA49941.6985990129486712No Hit
CATAATCACCCACAACAAATTTCCCGCCACCAAAATAAACCACCCCCTAA44981.5298955467046702No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC42651.4506457329247264No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA42451.4438431738019843No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA41251.4030278190655325No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA39491.343165298785403No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA38011.2928263612771125No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC37121.2625549731809107No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA31771.0805865166475628No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT30661.0428323135163449No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACACACACTTCAAT29791.0132411813324174No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT27360.9305900879911023No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA25900.8809314063950859No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA25730.8751492311407552No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC25620.8714078236232471No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC25540.8686867999741502No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC25400.863925008588231No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA24470.8322931086674806No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA23090.7853554507205611No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG21520.7319553616070366No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA18950.6445424768798022No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC17920.609509297397681No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT17840.6067882737485842No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA17600.5986252028012938No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT15980.5435244739070838No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT15420.5244773083634063No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC15240.5183550051529385No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA14610.4969269439163013No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC12940.4401255752414058No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT11290.3840044624787845No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA11140.37890254313672805No Hit
AAAAAAAATCCCAAAACAAACATACAAAATCAAAAAAATACCTAAAATAA10950.37244011197012317No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC10500.3571343539439537No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA10500.3571343539439537No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCAAAACCCAAAAAA9960.3387674443125504No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCG9780.3326451411020826No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC8700.29591132183927593No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC7390.251354559585316No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC6680.22720547469958197No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC6000.20407677368225927No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT5920.20135575003316247No Hit
AAAAAAATCCTTCCAACCAATAAAACTAACAATACCTACCCAACATGTAG5310.18060794470879946No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA4800.16326141894580742No Hit
CAACCATAAATACACAAAAAACAACCCTTCTTGTAGAACATCCTCCCCTA4070.1384320781477992No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4010.13639131041097663No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA3930.1336702867618798No Hit
CCTAAAAAAACAACACTCCAAACACCACTCTATCTTACAACCTAAATGTA3280.11156196961296841No Hit
CAACTACCAAACCCTAAAAAACCACAAACACTCAACACCAACCCATAAAA3260.1108817137006942No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACGCCAACAT3190.1085008180077345No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA2980.10135813092885543No Hit
AAACCTATCAAAACCTCCCCACAAATCTCTATCTTCCACCCTAATGTAGA2980.10135813092885543No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)