FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346774
Sequences flagged as poor quality0
Sequence length301
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT149574.313183802707239No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG128003.6911648508827075No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT106483.0705877603280523No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT102232.9480295523885873No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA93622.69974104171593No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA89682.586122373649697No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC68901.9868848298892072No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG65041.875573139854776No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA63601.8340475352823453No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA61861.7838707630906585No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC60291.7385963192165503No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC58111.6757311678499542No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA57391.654968365563739No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA52591.5165496836556374No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT50901.4678147727338267No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC50901.4678147727338267No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA46651.3452565647943617No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA46201.3322798133654772No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC41671.2016471823147064No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT39981.1529122713928956No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT38151.1001401489154319No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC35411.021126151326224No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA33220.9579726277056527No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA32030.9236563294826025No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC31760.9158702786252718No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCA30720.8858795642118498No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC30310.8740563017988661No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA27080.7809120637648728No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA25220.7272748245254834No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC24900.7180469123982767No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT24690.7119910950647973No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA24680.711702722810822No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC24080.6944003875723094No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA23790.6860375922070282No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA23480.6770980523337967No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACGCCAAACCCCCACAAAAC23150.6675817679526147No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA20360.5871259090935307No Hit
AAAAAAATCCTTCCAACCAATAAAACTAACAATACCTACCCAACATGACA19320.5571351946801086No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC17840.5144561010917773No Hit
TAATCTAAACCTCCTAACCTCAAACCATAAAAAAATCATCTACCCTCATG14870.4288095416611395No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA14770.4259258191213874No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA14700.42390721334356096No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA14700.42390721334356096No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC14190.4092002283908252No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT12670.365367645786593No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC11390.3284559972777659No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTTAAACCTCAACAAA11300.325860646991989No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA11250.32441878572211297No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCAAAACCCAAAAAA10850.3128838955631045No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC10360.29875365511831914No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA10180.29356295454676534No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC9790.2823164366417321No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA9680.2791443418480048No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA9050.26097688984756645No Hit
AAAAAAATCCTTCCAACCAATAAAACTAACAATACCTACCCAACATGTAG9040.26068851759359124No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT9020.2601117730856408No Hit
ACTCTAAAAAACCAACTCCTCCAAAAAATTCTATATATTTAAAAATCCAT8680.2503071164504836No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC7660.22089314654501202No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCAATCCACTC7180.20705127835420187No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT6950.20041871651277202No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC6780.1955163881951934No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC6550.18888382635376355No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA5730.1652373015277962No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC5030.1450512437495314No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTGA4030.11621401835201024No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACGCCTCCAT3800.10958145651058038No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT3510.10121866114529923No Hit
CCTAAAAAAACAACACTCCAAACACCACTCTATCTTACAACCTAAATGTA3470.10006517212939839No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)