FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences313881
Sequences flagged as poor quality0
Sequence length301
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT182715.820995855117066No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA181955.796782857197473No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG158205.040126672210169No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT151404.823484059245383No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA121753.878858548303338No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC102033.2505949707054587No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC90842.8940904355472297No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG88202.8099821269844303No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT86482.755184289587455No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC79402.529621098441766No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC70282.2390651234066414No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA68912.195418008735795No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA56771.808647226178074No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC46161.47062103153743No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT45191.4397175999821588No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA41341.3170596499947431No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA40531.2912536916857025No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT39771.2670406937661087No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA39491.258120115585206No Hit
AAAACAAAAAAAACTCCTAATCCCTCCTACGAATTTCTATATTCAAATAT36861.174330399100296No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA36381.1590379793616052No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT35921.144382743778693No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT29360.9353863406832527No Hit
TAAAACTAAACCCCACATACCTTCCTAACCCTAAACTATCCATCCTTCCA25460.81113543030639No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC23880.7607978819998662No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA23530.7496471592737375No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA18700.5957671856531616No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC18390.5858908312385904No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACACCTA14830.47247205150996713No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT13430.4278691606054524No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT12950.4125767408667616No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC12860.4097094121657571No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA11090.35331861437933487No Hit
ACTCTAAAAAACCAACTCCTCCAAAATACCCCTCTTAACTACCTTCCTTG10190.32464532736928964No Hit
AAAACCCCACTCTATCTTACAACCTAAATATCCCCTGTAGAACCATGTCG9900.3154061571104973No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA9530.30361825022858985No Hit
AAACCTATCAAAACCTCCCCACAAATCTCTATCTTCCACCCTAATGTAGA9220.29374189581401866No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGGTACCTT8960.28545850178889454No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC8740.27844947607532794No Hit
TCCAACCAATAACACTATCTCTAACCTAAACCTATAAACTCACAAAATCA8360.266342977115531No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA7980.2542364781557342No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC7680.24467871581905243No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC6760.21536824465322843No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT6040.1924296150451923No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC5500.17522564283916517No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC5460.1739512745276076No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTATCCGAAACCCAAAAAA5370.17108394582660308No Hit
CCTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA5350.1704467616708243No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA4340.13826896180399578No Hit
TAAAACTAAACCCCACATACCTTCCAAATCATAAAACACAACCAATTCCT3910.12456950245475196No Hit
AACCACTCACCTACTTAATCTCCCAATTCCAATATATCCAAAACTTACCC3900.12425091037686258No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA3800.12106498959796866No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA3490.11118863518339753No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC3340.10640975401505666No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)