FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000888439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422579
Sequences flagged as poor quality0
Sequence length301
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG177754.2063140856502566No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT165043.9055419223387817No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT139183.2935853414391154No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA122052.8882173510751836No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA117032.7694229954635703No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC95152.2516499873396456No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA93592.214733813085837No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT92852.1972222945295434No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA85922.0332292896712802No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC72111.706426490668017No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA71261.6863119085425446No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC70461.6673805371303352No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC59641.4113337387802045No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC58531.385066460945764No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA56951.3476770024066507No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT54181.2821271288918759No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA48711.1526838768608947No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA48521.148187676150495No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC48511.1479510340078425No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA48091.1380120640164324No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA47511.1242868197425806No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC44161.0450117019539542No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT39990.9463319284678131No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT38970.9221944299172462No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA38330.9070493327874789No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG36010.8521483556920718No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA34500.8164153921515267No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC33350.7892015457464757No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA31740.7511021607794046No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA30630.7248348829449641No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA29390.6954912572560398No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC28580.6763232437011778No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC26770.6334910158810542No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT23490.555872393090996No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA20640.4884293824350003No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT19870.4702079374507489No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT17350.4105741175022895No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC15890.37602436467500755No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA15660.3705815953939973No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA14880.3521235082670932No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC14200.3360318425667153No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA14190.33579520042406275No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC12990.3073981433057487No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT12880.30479507973657No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCGCAAAAC11490.2719018219078563No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCG11150.26385598905766733No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC10700.2532070926382996No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC10500.24847424978524726No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAGGGCGACCATCAGACA9620.22764974123181703No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACGT9250.21889398195367019No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA9240.21865733981101756No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA9010.21321457053000742No Hit
AAAACCCCACTCTATCTTACAACCTAAATATCCCCTGTAGAACCATGTCG8800.20824508553430246No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT8760.20729851696369198No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT8720.20635194839308152No Hit
AAACTACAAAATCAAAACCAACCCTTCCTTAAAAACCCGTCTAAACACAA7870.18623736626760914No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA7560.17890145984537803No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA7470.1767716805615045No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT7070.16730599485539982No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACGCCAAACCCCCGCAAAAC6140.1452982755887065No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACACCAAACCCCCACAAAAC6000.14198528559156987No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA5900.1396188641650437No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCGCACCAACAT5710.13512266345464397No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC5380.12731347274710764No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCGATTCACCCA5320.12589361989119194No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC5040.11926763989691869No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA5030.11903099775426607No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC4830.11429815490121373No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC4720.11169509133203495No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC4460.10554239562306693No Hit
AACCTCCTCTCCAAAACTAAATCTTACACGCCCCCAAAATTAATTTATAC4290.10151947919797245No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)