FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314577
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG135654.312139794072675No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT134154.264456714890154No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT113803.6175562739806155No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA81802.600317251420161No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT81652.595548943501909No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA78312.4893746205221614No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA67022.130479977875051No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC64062.0363853682882094No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC60841.9340256916430636No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC60331.9178134447210065No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT57021.8125927833249094No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA55041.7496511188039812No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA51401.6339401799877296No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG49091.5605082380466468No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA47661.5150503692259765No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC47651.5147324820314263No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC46231.469592500405306No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA45281.4393932169230426No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA42961.3656433877874097No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC42161.3402124122233985No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT39851.2667804702823158No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA38581.2264087965744475No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC37441.1901696563957316No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA34591.099571805948941No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC30770.9781388976307868No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA27860.8856337240166955No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA27290.8675141539273373No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA25590.8134733308538132No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC25280.8036188278227588No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA22500.7152461877378193No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT21430.6812322579209541No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC20670.6570728311351434No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA19760.6281450964310805No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCGCAAAAC19020.6046214440343699No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC16340.519427675894932No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA16000.5086195112802271No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT15900.5054406393347257No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA14400.45775756015220437No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACAACCATCAAACA13510.42946559983724175No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT13480.4285119382535913No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA13200.4196110968061873No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT12720.40435251146778056No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC12700.4037167370786803No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA12370.39322645965852554No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA12140.38591505418387234No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC11870.37733209993101846No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC11100.35285478595065756No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT10420.3312384567212479No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCACTCTAACCACCCTCG9790.31121156346458895No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA9690.3080326915190875No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC9580.304535932379036No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAGAAACAAATCCCGCATCG9360.2975424140989329No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC8960.2848269263169272No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC8920.2835553775387266No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC8640.2746545360913226No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC7680.24413736541450903No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTGTCAGCTAAAAACAAAA7590.24127638066355775No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA7410.23555441116165518No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCACCT6580.20916977401399342No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT6330.20122259415023985No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA6120.19454696306468688No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA5980.1900965423409849No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA5880.18691767039548346No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT5650.17960626492083018No Hit
TACTATCCACACTCTCTTACCTCCCACCATCGCTAACAACTACAACAAAA5590.17769894175352932No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA5540.17610950578077864No Hit
ATAATACTCCTTTATCCCAATCCCCAATCCCAAAACTCAACAACCTAAAC5210.16561922836062395No Hit
TAAAACTAAACCCCACATACCTTCCCCCAAAAAAACCTACTCATAAACTG5200.1653013411660738No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC5190.16498345397152367No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCAAAACCCAAAAAA5160.16402979238787324No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAACAACATT5080.1614866948314721No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT5030.15989725885872139No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4940.15703627410777013No Hit
ACCCCCAAAAAAACCTACTCATAAGCGCTTAAAACCCCCACCACCCGCAA4920.15640049971866984No Hit
ATACAAAACAAACCCCTATCTCCTCCACCGCGACCTTAAAAAACAATACC4810.15290374057861827No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAGCCGCCCCCTAA4810.15290374057861827No Hit
AAAACCCCACTCTATCTTACAACCTAAATATCCCCTGTAGAACCATGTCG4330.13764515524021145No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATGA4240.13478417048926017No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACACTACCTT3560.11316784125985054No Hit
CAAAAAAACAACCTTCTCTCAATAAAGAAAAACAAAAACACCTAACGCTA3530.11221417967620011No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA3520.11189629248164996No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC3440.10935319492524882No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCGCC3340.1061743229797474No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA3270.10394911261789642No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC3170.100770240672395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)