FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275756
Sequences flagged as poor quality0
Sequence length301
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA145915.2912719940817246No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG131494.768345929009704No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT126314.580498701750823No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA86193.1255892890816517No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA85823.1121716299917317No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT84103.0497976471953465No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC70452.5547948186077547No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA66872.4249699009269063No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC64132.325606695774525No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA60042.1772871669156793No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC59452.1558914402587797No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC57732.0935174574623945No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA55312.0057587142256197No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC54491.976022280566878No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA53191.9288791540347263No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA50791.8418456896676771No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA39051.416106993138862No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT33901.229347684184569No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA33831.22680920814053No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA32581.181479278782692No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA32051.1622593887349686No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC31971.159358273256067No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC30611.110039310114739No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA30451.1042370791569358No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC27440.9950826092632618No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC27430.994719969828399No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA27100.9827528684779298No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC25380.9203788856815446No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA22340.8101364974832823No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA17460.6331684532702825No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATGA17110.6204760730500878No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA16370.5936407548702476No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACGCCAAACCCCCACAAAAC14810.5370690030316657No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC14490.5254645411160591No Hit
AAACTACAAAATCAAAACCAACCCCCTAATACCTTGTAGAACCATGTCGT12330.44713442318571495No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT12080.4380684373141473No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT12080.4380684373141473No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT12000.43516732183524565No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC11930.4326288457912067No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA10990.39854073891411246No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC10790.39128795021685836No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC10550.38258460378015346No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACACTA9330.3383425927269035No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT8350.3028039281103584No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC8210.29772697602228054No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA6650.2411552241836986No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT6550.2375288298350716No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC6100.2212100552662499No Hit
AAACCTATCAAAACCTCCCCACAAATCTCTATCTTCCACCCTAATGTAGA5790.20996823278550603No Hit
AAACTACAAAATCAAAACCAACCCTAATACCTTGTAGAACCATGTCGTCA5750.2085176750460552No Hit
AAACTACAAAATCAAAACCAACCCCACGTACCCCATACAACTCCCTAATA5240.19002306386805728No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT4920.1784186019524507No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCGAACGCT4880.1769680442129999No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4270.1548470386863749No Hit
CCTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA3590.130187557115711No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA3050.11060502763312495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)