FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274264
Sequences flagged as poor quality0
Sequence length301
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA215997.8752588746609105No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG173206.31508327742613No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT169666.186010559169267No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT123594.506242160837734No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA108213.945468599597468No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC107823.931248723857305No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT102003.719044424350261No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC98123.5775748913455647No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC87453.1885336755826508No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG72762.6529183560365195No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA64592.3550301898900328No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA45991.676851500743809No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC44061.6064813464399266No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC42631.5543418020593298No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC38691.4106845958638392No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT34111.2436922089665432No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT33081.206137152524575No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA29391.0715952512907272No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCGATCCACTC24340.8874660910655428No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC22440.8181897733570574No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT16070.585931802934399No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC12030.4386284747542514No Hit
AAAACCCCACTCTATCTTACAACCTAAATATCCCCTGTAGAACCAAAAAA11850.43206545518186856No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC11490.418939416037103No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC7560.2756468220400782No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA7180.2617915584983811No Hit
AACTTACACATCTCAAAAAAACCCAATCTCACCTAAAAATGTAGAACCAT6720.24501939736895836No Hit
CCTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA6230.22715339964413847No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT6190.22569495085027566No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA5920.21585042149170142No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCCCCGCCT5810.21183968730857858No Hit
AACTTACACATCTCAAAAAAACCCAAATCCCTAATCTCACCTAAAAATGT4500.16407548930957033No Hit
AAAACAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACAA3600.13126039144765628No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT3230.11776974010442494No Hit
AAACTACAAAATCAAAACCAACCCATACAACTCCCTAATACCTTGTAGAA3000.10938365953971356No Hit
TACTATCCACACTCTCTTACCTCCCGACCCCCACACTCCCTTCTAACACC2870.10464370095965932No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)