FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314891
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT137684.372306607683293No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT104693.324642495339657No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG88492.8101787602694266No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA87182.7685770631742415No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA72212.293174463544528No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA67002.1277203857842872No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA66402.108666173374279No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT64842.0591252211082565No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC61901.9657595802992145No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT58511.8581032801826665No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG58461.8565154291484989No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA55791.771724183923961No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC54891.743142865308948No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC53731.7063047213162648No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC51711.6421555395359029No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC50231.5951551489245486No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA50041.5891213149947125No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA48681.5459317668653598No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA40871.2979094353284153No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA35511.1276918044656723No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA33781.072752158683481No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA33531.0648129035126441No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC30400.9654134287737661No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA30060.954616041741428No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC29930.9504876290525929No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA29650.9415956632612554No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA28940.9190481785760787No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT26800.851088154313715No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC26350.8367974950062085No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC25960.8244122569397029No Hit
TAAAACTAAACCCCACATACCTTCCCCAACCCAAACACAACACTAACACA24620.7818578492240171No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT24210.7688374707438447No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA24080.7647090580550095No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC23620.7501008285406696No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT21070.6691204257981334No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA21050.6684852853844664No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC20200.641491817803621No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACGAACATTAAAACCTTCATC16930.5376463601690744No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT16770.5325652368597388No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC16430.5217678498274007No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAGACA15250.4842945654210505No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC14220.4515848341172025No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT13220.4198278134338549No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA13130.4169696815723536No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA12790.4061722945400154No Hit
AAACCTATCAAAACCTCCCCACAAATCTCTATCTTCCACCCTAATGTAGA12020.3817193886138378No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA11680.3709220015814996No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC11350.36044218475599493No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA11300.35885433372182757No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA10480.33281357676148254No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC9670.307090390007971No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC9060.287718607391129No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT8650.2746982289109565No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTACAC8520.2705698162221213No Hit
CCCAAAAATTAATAAATAAACCCCAAATTAATAAATAAACCCCAAATTAA7780.2470696209164441No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC7120.22610998726543471No Hit
CCCAAAAATTAATAAATAAACCCCAAATTAATAAATAAACCACTCACCTA6340.20133951113242363No Hit
ATAATACTCCTTTATCCCAATCCCCATAATCCCAACCACCACAACCCCAC5540.17593389458574554No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCG5500.17466361375841163No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTCCACCAAAAATACTAAA5180.16450136713974042No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA5090.16164323527823912No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC4730.150210707832234No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT4720.14989313762540055No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4530.1438593036955645No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCGAAACCCAAACGCCTCCAT4220.13401462728372676No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC4170.13242677624955937No Hit
AACTTACACATCTCAAAAAAACCCATGTCGTCAGTGTCCAAATCCCTAAT4100.13020378480172504No Hit
AAACTACAAAATCAAAACCAACCCAACTAAATAAAAACTTACCCAACCCC4050.12861593376755764No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA3980.12639294231972334No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA3500.11114957239171651No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC3360.10670358949604784No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT3300.10479816825504698No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)