FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289728
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT124984.31370112657389No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG121544.194969074442236No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT87713.027322178042854No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA80002.76121051468964No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT74572.5737933510050803No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA69752.40743041749503No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA60842.099900596421471No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA58392.015338524409101No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA56421.9473437154848685No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC54121.8679589131875414No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC52981.8286116633532141No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC52511.8123895515794124No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT51211.7675198807157055No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA51141.7651038215153525No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC45651.5756157499447758No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA45451.5687127236580518No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC40761.4068367572343716No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA38761.3378064943671306No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA35571.227703225093881No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC34441.18870112657389No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA30771.0620305942125028No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA30041.0368345482659598No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA29421.015435166777115No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC29211.0081869891760546No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT26740.9229346145350122No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT25680.8863485752153745No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA25540.8815164568146676No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC24970.8618428318975038No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA23430.8086895294897282No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG20640.7123923127899271No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA20590.7106665562182461No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT19700.6799480892423239No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC17400.6005632869449967No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA16400.5660481555113761No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC16110.5560387673956262No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC15790.5449939253368676No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT14210.49046001767174724No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT12900.4452451954937044No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA12580.43420035343494584No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC12190.42073945217583386No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA11580.3996852220013254No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA11410.39381764965760985No Hit
AAACCTATCAAAACCTCCCCACCAGGAACCTACCGCTCTAACCACCCTCG11310.39036613651424784No Hit
CCTAAAAAAACAACACTCCAAACACCACTCTATCTTACAACCTAAATGTA10410.3593025182239894No Hit
TAAAACTAAACCCCACATACCTTCCCCAACCCAAACACAACACTAACACA9310.32133587364700683No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC8990.3102910315882483No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC8990.3102910315882483No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTACAA8970.3096007289595759No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC8320.28716589352772254No Hit
ACTCCCACACACACTAACACAATAAAACAACACTACGAACATCAATACAC8150.2812983211840071No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC7530.2598989396951623No Hit
CAACTACCAAACCCTAAAAAACCACGTCGTCAGTGAAACAAAACTATCTC7510.25920863706648994No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC7490.2585183344378175No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACACCAAACCCCCACAAAAC7440.2567925778661365No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCGATCCACTC7440.2567925778661365No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACAAACATCAATACAC7340.25334106472277446No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC7290.25161530815109345No Hit
CCTAAAAAAACAACACTCCAAACACTCTATCTTACAACCTAAATGTAGAA7060.24367682792136072No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT6950.23988016346366248No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA6930.23918986083499008No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA6790.2343577424342832No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT6370.2198613872321626No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA6280.21675502540313674No Hit
AAAAAAATCCTTCCAACCAATAAAACTAACAATACCTACCCAACATGTAG6080.20985199911641267No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC6040.2084713938590678No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA5880.20294897282968852No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT5570.19224928208526618No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCACTTACCAATCCACTC5560.19190413077092996No Hit
TAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCGCCT5420.1870720123702231No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA5400.1863817097415507No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC5120.17671747294013695No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCG4910.16946929533907665No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC4840.16705323613872322No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCGCACTCCCTTCTAACACC4620.1594599072233267No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA4250.14668930859288712No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA4120.14220234150651648No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4000.138060525734482No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC3820.13184780207643032No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA3680.12701568367572344No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACGAACC3640.12563507841837862No Hit
CATAATCACCCACAACAAATTTCCCACCGCCAAAATAAACCACCCCCTAA3570.12321901921802517No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC3530.12183841396068036No Hit
CAACTACCAAACCCTAAAAAACCACTCTACACTAACCGAAACCCAAAAAA3470.11976750607466313No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATGCC3450.11907720344599072No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATAATAAA3320.11459023635962005No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAGTGCC3000.1035453943008615No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCAAAACCCAAAAAA2940.10147448641484426No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)