FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58650
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGGGTTAGTTATAGGGGAGGATACACTGACGACATGGTTCTACAAGA3143353.594202898550726No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTAGTTATTTTAGTTATTTTAGTT53739.161125319693095No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTATGAAGGGTTTTGGGTTT15712.678601875532822No Hit
AGAAGGGTTGTTTTTTGTGTATTTATGGTTGAGACCAAGTCTCTGCTACC13852.361466325660699No Hit
AGAAGGGTTAGTTATAGGGGAGGATACATTGACGACATGGTTCTACAAGA11331.9317988064791136No Hit
AGGTATTAGGGAGTTGTATGGGGTTGGTTTTGATTTTGTAGTTTAGACCA7111.2122762148337596No Hit
AGAAGGGTTAGTTATAGGGGAGGATACACTGAGGACATGGTTCTACAAGA4130.7041773231031543No Hit
AGAAGGGTTAGTTATAGGGGAGGTTACACTGACGACATGGTTCTACAAGA2810.4791133844842285No Hit
AGAAGGGTTAGTTATAGGGGAGGATACATTGAGGACATGGTTCTACAAGA2740.46717817561807334No Hit
AGAAGGGTTAGTTATAGGGGAGGATACGCTGACGACATGGTTCTACAAGA2170.36999147485080985No Hit
AGAAGGGTTAGTTATAGGGGAGGATACACTGACGACATGGTTCTACGAGA2170.36999147485080985No Hit
GTTAAAAGAATTTGGAAGGGTTTGATATTATGTTGAGTTTTAGTTGGAGA1710.2915601023017903No Hit
AGAAGGGTTAGTTATAGGGGAGGATACACTGACGACATGGTTCTGCAAGA1520.2591645353793691No Hit
AGAAGGGTTAGTTATAGGGGAGGATTCACTGACGACATGGTTCTACAAGA1510.25745950554134694No Hit
AGAAGGGTTAGTTATAGGGGAGGATACACTGACGACGTGGTTCTACAAGA1410.2404092071611253No Hit
AGAAGGGTTAGTTATAGGGGAGGATAGACTGACGACATGGTTCTACAAGA1350.23017902813299232No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTGGAGTGTTGTTTTTTTAGGAGAC1340.22847399829497017No Hit
AGAAGGGTTGGTTATAGGGGAGGATACACTGACGACATGGTTCTACAAGA1300.2216538789428815No Hit
AGAAGGGTTAGTTATAGGGGAGGATACACTGACGACATGGCTCTACAAGA1300.2216538789428815No Hit
AGAAGGGTTAGTTATAGGGGAGGATACACTGACGACATGGTTCTACTAGA1290.21994884910485932No Hit
AGAAGGGTTAGTTATAGGGGAGGTTACATTGAGGACATGGTTCTACAAGA1260.21483375959079282No Hit
TTAGGGTGGAAGATAGAGATTTGTGTTTATAGGGTTTAGGAAGGCGGGTA1230.20971867007672632No Hit
AGAAGGGTTAGTTATAGGGGAGGATACACTGACGACATGGTCCTACAAGA1160.1977834612105712No Hit
AGAAGGGTTAGTTATAGGGGAGGATACACTGATGACATGGTTCTACAAGA1070.1824381926683717No Hit
AGAAGGGTTAGTTATAGGGGAGGATAGATTGAGGACATGGTTCTACAAGA1000.17050298380221654No Hit
AGGTATTAGGGAGTTGTATGGGGTACACTGACGACATGGTTCTACAAGGT950.16197783461210571No Hit
GGGAGATTAAGTAGGTGAGTGGTTTATTTATTAATTTTTGGGAGACCAAG940.16027280477408354No Hit
AGAAGGGTTAGTTATAGGGGAGGATACACTGACAACATGGTTCTACAAGA870.1483375959079284No Hit
AGAAGGGTTAGTTATAGGGGAGGATACACTGACGACATGGTTCTATAAGA850.14492753623188406No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTAGTTTTTTTAGTTATTTTAGTT810.1381074168797954No Hit
AGAAGGGTTAGTTATAGGGGAGGATACACTGACGGCATGGTTCTACAAGA790.13469735720375106No Hit
AGAAGGGTTAGTTATAGGGGAGGTTACATTGACGACATGGTTCTACAAGA790.13469735720375106No Hit
TTTAGGTTGTAAGATAGAGTGGTGTTTGGAGTGTTGTTTTTTTAGGAGAC760.12958226768968456No Hit
AGAAGGGTTAGTTATAGGGGAGGATAGATTGACGACATGGTTCTACAAGA760.12958226768968456No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTGGTTATTTTAGTTATTTTAGTT720.1227621483375959No Hit
AGAAGGGTTAGTTATAGGGGAGGATACACTGGCGACATGGTTCTACAAGA700.11935208866155159No Hit
AGAAGGGTTAGTTATAGGGGAGGATAGACTGAGGACATGGTTCTACAAGA690.1176470588235294No Hit
AGAAGGGTTGTTTTTTGTGTATTTATGGTTGAGACCAAGTCTCTGCTATC650.11082693947144075No Hit
AGAAGGGTTGTTTTTTGTGTATTTATGGTTGAGACTAAGTCTCTGCTACC630.10741687979539641No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTATGAAGGGTTTTGGGTTTT620.10571184995737425No Hit
AGAAGGGTTAGTTATAGGGGAGGATACACTGACGACATGGTTTTACAAGA620.10571184995737425No Hit
AGAAGGGTTAGTTATAGGGGAGGATATACTGACGACATGGTTCTACAAGA590.10059676044330776No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)