FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58650
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAATACACAAAAAACAACCCTTCTTGTAGAACCATGTCGTCAG3013451.37936913895993No Hit
AATACCTTAACTCCCTAAAACCCAAAAATAACTAAAATAACTAAAATAAC54519.294117647058824No Hit
AATACCTAAAAACCCAAAACCCTTCATAAAAAAACCCAACATAAAAAAAC16352.78772378516624No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA13542.308610400682012No Hit
CAACCATAAATACACAAAAAACAACCCTTCTTGTAGGACCATGTCGTCAG8941.5242966751918159No Hit
CAACCATAAATACACAAAAAACAACCCTTCTTGTAGAGCCATGTCGTCAG3020.5149190110826939No Hit
CAACCATAAATACACAAAAACAACCCTTCTTGTAGAACCATGTCGTCAGT2660.453537936913896No Hit
CAACCATAAATACACAAAAAACAACCCTTCTCGTAGAACCATGTCGTCAG2510.42796248934356357No Hit
TAACCATAAATACACAAAAAACAACCCTTCTTGTAGAACCATGTCGTCAG2240.38192668371696503No Hit
CAACCATAAATACACAAAAAACAACCCTTCTTGTAGAACCATGTCGTCGG2040.34782608695652173No Hit
CAACCATAAATACACAAAAAACAACCCTTCTTGTAGAACCATGTCGCCAG1910.3256606990622336No Hit
CAACCATAAATACACAAAAAACAACTCTTCTTGTAGAACCATGTCGTCAG1900.32395566922421143No Hit
CAACCATAATACACAAAAAACAACCCTTCTTGTAGAACCATGTCGTCAGT1870.3188405797101449No Hit
AATACCTTAACTCCCTAAAACCCAAGAATAACTAAAATAACTAAAATAAC1840.3137254901960784No Hit
CAACCATAAATACACAAAAAACAACCCTTCTTGTAGAACCACGTCGTCAG1820.3103154305200341No Hit
CCAACTAAAACTCAACATAATATCAAACCCTTCCAAATTCTTTTAACTGT1760.30008525149190113No Hit
CCTAAAAAAACAACACTCCAAACACCACTCTATCTTACAACCTAAATGTA1750.29838022165387895No Hit
CAACCATAAATACACAAAAAACAACCCTTCTTGTAGAACCATGCCGTCAG1700.2898550724637681No Hit
CAACCATAAATACACAAAAAACAACCTTCTTGTAGAACCATGTCGTCAGT1630.27791986359761295No Hit
CAACCATAAATACACAAAAAACAACCCTTCTTGTAGAACCGTGTCGTCAG1360.2318840579710145No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG1230.20971867007672632No Hit
CAACCATAAATACACAAAAAACAACCCTTCTTGTAGAACCATGTTGTCAG1230.20971867007672632No Hit
CCTAAAAAAACAACACTCCAAACCCCACTCTATCTTACAACCTAAATGTA1150.19607843137254902No Hit
CAACCATAAATACACAAAAAACAACCCTTCTTGTGGAACCATGTCGTCAG1150.19607843137254902No Hit
CAACCATAAATACACAAAAAACAACCCTTCTTGCAGAACCATGTCGTCAG1150.19607843137254902No Hit
CAACCATAAATACACAAAAAACAACCCTCCTTGTAGAACCATGTCGTCAG1010.1722080136402387No Hit
CAACCATAAATACACAAAAAACAACCCTTCCTGTAGAACCATGTCGTCAG980.1670929241261722No Hit
CAACTATAAATACACAAAAAACAACCCTTCTTGTAGAACCATGTCGTCAG950.16197783461210571No Hit
CATACCTTAACTCCCTAAAACCCAAAAATAACTAAAATAACTAAAATAAC950.16197783461210571No Hit
CAACCATAAATACACAAAAAACAACCCTTCTTGTATAACCATGTCGTCAG930.1585677749360614No Hit
CAACCATAAATACACAAAAAACAACCCTTCTTGTAGAACTATGTCGTCAG910.15515771526001704No Hit
CAACCATAAATACACAAAAAACAACCCTTCTTGTAGAACCATTTCGTCAG910.15515771526001704No Hit
CAACCATAAATACACAAAAAACAACCCCTCTTGTAGAACCATGTCGTCAG880.15004262574595056No Hit
CAACCTAAATACACAAAAAACAACCCTTCTTGTAGAACCATGTCGTCAGT870.1483375959079284No Hit
CAACCATAAATACACAAAAAACAACCCTTCTTGTAGAACCATGTCGTTAG860.1466325660699062No Hit
CAACCATAAATACATAAAAAACAACCCTTCTTGTAGAACCATGTCGTCAG840.14322250639386191No Hit
CCCAAAAATTAATAAATAAACCCCACCTACTTAATCTCCCTGTAGAACCA800.13640238704177324No Hit
CAACCATAAATACACAAAAAACAACCCTTCTTGTAGAACCATGTCATCAG780.1329923273657289No Hit
AATACCTTAACTCCCTAAAACCCAAAATAACTAAAATAACTAAAATAACT770.13128729752770674No Hit
CAACCATAAATACACAAAAAACAACCCTTCTTATAGAACCATGTCGTCAG760.12958226768968456No Hit
CAACCATAAATACACAAAAAACACCCCTTCTTGTAGAACCATGTCGTCAG710.12105711849957373No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA690.1176470588235294No Hit
CAACCATAAATACACAAAAAACAACCCTTCTTGTAGAATCATGTCGTCAG630.10741687979539641No Hit
AATACCTAAAAACCCAAAACCCTTCATAAAAAACCCAACATAAAAAAACC620.10571184995737425No Hit
CAACCATAAATACACAAAAAACAACACTTCTTGTAGAACCATGTCGTCAG610.1040068201193521No Hit
AAAAACCACAATAACCTTCTCAAAAAACCCTTTCTGTAGAACCATGTCGT610.1040068201193521No Hit
AATACCTTAACTCCCTAAAACCCAAAAAATAACTAAAATAACTAAAATAA610.1040068201193521No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)