FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284843
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG130514.5818222670032265No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT128964.527406325589886No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT110983.8961814051951427No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA83142.9188008832936037No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA81482.8605231653928658No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA67132.3567368690822663No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC65322.2931930923350756No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT64602.267916009872105No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC64162.252468903922512No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC59982.1057213974013753No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA59292.081497526707695No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA58632.0583268677833053No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA57102.004613067549492No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC56931.998644867523513No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT54571.9157922083393306No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA52161.8311841962063313No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA51091.7936196431016385No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC49521.7385015605087717No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA42651.4973160653412583No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC40451.4200805355932917No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA39951.4025270061051176No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA31451.1041170048061564No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA29941.0511053457518702No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC26000.9127835333850578No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC25420.8924214391787757No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA22550.7916641799166558No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG22410.786749191659967No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA22320.7835895563520956No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT22210.7797277798646974No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT20810.7305778972978096No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA18140.6368420498309596No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT17710.6217460144711296No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC17290.6070010497010634No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC15700.5511808259286695No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT15050.5283612375940431No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA14520.5097544963365783No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT12980.4556896255130019No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA11430.4012736840996619No Hit
CAACTACCAAACCCTAAAAAACCACTACCCCGCCTAACAAAACCCCAAAA10270.3605494956870978No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA10240.35949628391780736No Hit
CAACTACCAAACCCTAAAAAACCACTACACAAAAAATTCCTATTCAAAAC9150.3212295896335876No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC9010.3163146013768989No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT8130.28542038947771226No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT7780.27313291883599033No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC7240.2541751069887622No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA7180.25206868345018135No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC7040.24715369519349256No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA6300.22117447155099476No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACGCGACC6170.21661055388406944No Hit
TAAAACTAAACCCCACATACCTTCCCAACTAAAATAATATCACCCCCAAA6160.216259483294306No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC5310.18641848316440987No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAGCCCCCACCACCCGCAA5140.18045028313843064No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA4710.16535424777860086No Hit
AAAACCCCACTCTATCTTACAACCTAAATATCCCTGTAGAACCATGTCGT4660.16359889482978343No Hit
TAAAACTAAACCCCACATACCTTCCCCAACCCAAACACAACACTAACACA4640.16289675365025647No Hit
CAACTACCAAACCCTAAAAAACCACTCTACACTAACCGAAACCCAAAAAA4630.162545683060493No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCAAAACCCAAAAAA4560.16008818893214857No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC3500.1228747064172193No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT3490.12252363582745582No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACGAACATTAAAACCTTCATC3440.1207682828786384No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCAATCCACTC3110.10918295341644343No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT3020.10602331810857209No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)