FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335032
Sequences flagged as poor quality0
Sequence length301
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTCGGAAGTATGGTATTT226576.762637598796532No Hit
GATTGTTTAGGGAGGGGGTTAATAGCGTGTTAGTATTGAGGTTAGGTTTG153454.5801595071515555No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTCGTTTGAGGTTTTTTGT131903.9369373671768666No Hit
TTAGGGTGGAAGATAGAGATTTGTGTTTATAGGGTTTAGGAAGGCGGGTA131523.925595167028821No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTTCGTTTATT111653.332517490866544No Hit
GTTAAAAGAATTTGGAAGGGTTTGTGAGATATTGGATTGCGGGGTAATAA110783.306549822106545No Hit
GTTGGGGATTGAGGTTTTTAGAGTAGAGGAGTTAGTTGTGGGTATTAGTG108673.2435707633897657No Hit
TGGTTTTTATTTTTGTGTTTTTTGGGGATGGTTTTTAAGGATAGTATGTT105533.1498483726927575No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGCGTCGGGTA81922.445139568757611No Hit
AGAAGGGTTAGTTATAGGGGAGGATGTGGTATAGGTGTTTATGGGATTTT79312.367236562477614No Hit
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTTGGAAGTATGGTATTT74132.2126244657226772No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGTGGTGTTGAT64281.9186226987272859No Hit
GTTGGTTTTTTTGGGGTGAAGCGGTTTTTATTTTCGTTGATGAACGGTTT64101.9132500776045274No Hit
GTGAAGTTTTTGGTTTTTTGTTGTATAGATGAGGTTGGGATGTAAGGAGA62511.8657919243534944No Hit
GTTTATGAGTAGGTTTTTTTGGGGTTGAGGAGGAGTTTGGTATTTGTTAG51161.5270183146684495No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGTGTTATTTTGTGATGGGTTAG48791.456278803218797No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTCGTT48321.4422502925093723No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTTCGTTATTTTACGATGAGTTAG48321.4422502925093723No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTCGT46661.392702786599489No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTTCGTTTATC46321.382554502256501No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTCGTGGTGGGGGGTAT43371.2945032116335156No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTAGTATTTTTGATTTTTTTGATT41701.244657226772368No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGACGTTGAGCGTGAAGAAGTGG30380.906779053941116No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTTAGTTTTATTTTTAAATT28550.8521574058597388No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTTATGGGATAGGGAGTAT28070.8378304161990497No Hit
AGAAGGGTTAGTTATAGGGGAGGATTGGGGTGATTGAGGAGGGTTTGGAA19710.5883020129420473No Hit
GGGTTTAGGTTGTTGAGTTTTGGGTTTGTTTTTTATGTGTAGCGGTTTGA18890.56382673893837No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTCGTTT18140.5414408175935432No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTAATTAATTGAGAAGTTGT16980.5068172592468779No Hit
GAATTTTGGGTTTGATTTTTGTTTGAGTGGAAGGAAGTTTTTATTAGGGG16760.500250722319062No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTAATTAATTGAGAAGTTGTT15400.4596575849471095No Hit
TGATTTTGTGAGTTTATAGGTTTAGGTTTTAGTAGTAGAATTATTTTGGT15160.452494090116765No Hit
TTTAAGGTTTGTGTTGATGTTAATGTTATAGGTTACGATAAGGTATGTTA14470.4318990424795243No Hit
TTGTGTGATGGGGATAGTTTATTTTGTAAGGTAGTTGAGGTATTTTGGGT13000.3880226366436639No Hit
TGTTGGGTAGGTATTGTTAGTTTTGTTGTATGGATGAAGGTGTGAAGGTT12420.3707108574703312No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTGGAGGAGTTGGTTTTTTAGAGTAG11440.34145992024642424No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTCG10840.3235511831705628No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTTGGGGTAAGTGTTTATTTTTTTT10810.32265574631676974No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTGATATTATTTTAGTTGGGAAGGTA10790.322058788414241No Hit
TTAGGGTGGAAGATAGAGATTTGTGGGGAGGTTTTGATAGGTTTAGACCA10170.3035530934358509No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTATTTTGCGATGGGTTAG9790.29221089328780536No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGCGGCGTTGAT9600.2865397932137826No Hit
GATAGGTTTGGAGATAGTTTTGTTTTTGTGGTTTTTTAGGGTTTGGTAGT8900.26564626662527757No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTAATTAATTGAGAAGTTGTTT8510.2540055875259677No Hit
GTTGGGGATTGAGGTTTTTAGAGTAGAGGAGTTAGTTGTGGGCATTAGTG8030.23967859786527854No Hit
GGGGTGTATTTATTAGTTTTAGGGATGTATTTTTAGGTTTCGGGGGTTTA7770.23191814513240527No Hit
AGGTATTAGGGAGTTGTATGGGGTATTGTGAAGTGGGTTGGTTTTGATTT7650.22833639771723296No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTCGTTTATTG7610.22714248191217554No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTTAATTAATTGAGAAGTTG7420.22147138183815276No Hit
TGTTGGGTAGGTATTGTTAGTTTTGTTGTATGGATGAAGGCGTGAAGGTT7340.2190835502280379No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTATGGGATAGGGAGTATT7330.21878507127677357No Hit
GGGAGATTAAGTAGGTGAGTGGTTGGGGTTTATTTATTAATTTTTGGGAG6670.19908546049332598No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTGGTGATGAAGGGTAAGGGAAAAGG4940.14744860192459228No Hit
GATTGTTTAGGGAGGGGGTTAATAGTGTGTTAGTATTGAGGTTAGGTTTG4910.1465531650707992No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGT4520.1349124859714893No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTAGTTTTATTTTTAAATTT4340.12953986484873087No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTTATTGGTTTTTGGAGTTTTTTTT4290.12804747009240908No Hit
GTTTATGAGTAGGTTTTTTTGGGGTTTTTTAGTTTTTGGAAGGTATGTGG4150.12386876477470808No Hit
TAGGTTTTATTGTGAGGATGATTTGGGTGATACGTTGTTGGTTGTTTTGA4120.12297332792091502No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTGGGTGGATATAAAGGTTTTAAAA4010.1196900594570071No Hit
TTTTTTTTGGGTTTTTAGGGTAGAGTTCGGGTTGGGGAGGTTAGTGTTTC3980.11879462260321401No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTGTGGGTTTTGGGATAAAGTTGGCG3890.1161083120418348No Hit
GTTAAAAGAATTTGGAAGGGTTTGTGTTTGGTGGTGGTTGGTTGTGGTTT3830.11431743833424868No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGCGGTGTTGAT3760.11222808567539819No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTTTCGTTTAT3610.10775090140643281No Hit
TAGGTTTTATTGTGAGGATGATTTGGGTGATATGTTGTTGGTTGTTTTGA3360.1002889276248239No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)