FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335032
Sequences flagged as poor quality0
Sequence length301
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT203946.087179732085294No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA138264.126769980180998No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA134314.0088707944315765No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT123943.6993481219704387No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG117913.5193653143580312No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA97312.9044986747534565No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT89632.6752668401824304No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG71962.147854533298312No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA65131.9439934095847562No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA62511.8657919243534944No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC58941.7592349387521193No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC58571.748191217555338No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC58491.7458033859452233No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC53001.581938441701091No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA52091.5547768571360348No Hit
TAAAACTAAACCCCACATACCTTCCAAAAAACCTAATTCCCAAAATAACA50731.5141837197640822No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT49041.4637407770004058No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC46851.3983738866735118No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC39771.187050789178347No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA39531.1798872943480025No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC36891.1010888512142123No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC35831.0694500823801907No Hit
AAACTACAAAATCAAAACCAACCCAACTAAATAAAAACTTACCCAACCCC35561.061391150696053No Hit
TAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCGCCT31510.9405071754339883No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA29540.8817068220349101No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCC22440.6697867666372167No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT20120.600539649943886No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA19090.5697963179636573No Hit
CAACCATAAATACACAAAAAACAACCAACACAATTCCAAACCCTCCTCAA18990.5668115284510137No Hit
CCCCTAATAAAAACTTCCTTCCACTCAAACAAAAATCAAACCCAAAATTC17800.5312925332505551No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT16770.5005492012703264No Hit
ATAATACTCCTTTATCCCAATCCCCATCAAACAAATACTCTATCAATAAC16300.48652069056090164No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC15420.4602545428496382No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA14170.42294467394159363No Hit
CACCCAAAATACCTCAACTACCTTACAAAATAAACTATCCCCATCACACA12400.3701138995678025No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT11930.3560853888583777No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACACACACTTCAAT11700.3492203729792975No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC11670.34832493612550447No Hit
AAACCTATCAAAACCTCCCCACAAATCTCTATCTTCCACCCTAATGTAGA11590.3459371045153896No Hit
TAAAACTAAACCCCACATACCTTCCCAACTAAAATAATATCACCCCCAAA11440.34145992024642424No Hit
ACTCTAAAAAACCAACTCCTCCAAAATACCCCTCTTAACTACCTTCCTTG10530.3142983356813677No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT9540.2847489195061964No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA8920.2662432245278063No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCCTAAAAAACAATACC8680.25907972969746174No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT8320.2483344874519449No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA7070.21102461854390028No Hit
AAACTACAAAATCAAAACCAACCCACTTCACAATACCCCATACAACTCCC6740.2011748131521765No Hit
CCCAAAAATTAATAAATAAACCCCAACCACTCACCTACTTAATCTCCCTG6490.1937128393705676No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC6340.18923565510160223No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA5260.1569999283650517No Hit
TAAAACTAAACCCCACATACCTTCCCCCCAACCAACAATAACCCAAAACA5010.14953795458344277No Hit
TAAAACTAAACCCCACATACCTTCCAAAAACTAAAAAACCCCAAAAAAAC4510.13461400702022494No Hit
AACTTACACATCTCAAAAAAACCCACATTCTATACCCTCACTCCCGAAAC4310.1286444279949378No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT4230.12625659638482295No Hit
AAAACAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACAA4180.12476420162850115No Hit
AAAAATCCTAAAAACCCCAAAACTCTTCCAATCAACAATAAAATTTCCTT4170.1244657226772368No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA3650.10894481721149024No Hit
CCCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA3600.10745242245516846No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT3530.10536306979631797No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)