FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000888467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400624
Sequences flagged as poor quality0
Sequence length301
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT165204.1235672351132235No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG123593.084937497503894No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT109492.7329865409960465No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA99532.4843743759734815No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT96232.402002875514198No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA91552.2851851112264865No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC76051.8982886696753065No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA74051.8483665481848317No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA71601.7872119493590002No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT69091.7245596868884538No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC66661.663904309277527No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA66041.6484284516154797No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC65571.6366967530652181No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC65031.6232177802627903No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA52231.3037162027237508No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA51101.2755102040816326No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC48061.199628579416111No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA46481.1601901034386357No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC41581.0378809057869725No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC41411.033637525460282No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA41111.0261492072367107No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA40020.998941651024402No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA38590.9632473341587124No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT37940.947022644674308No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA37180.9280522385079277No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA36710.916320539957666No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA35300.8811254443068812No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT32940.8222173409481208No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA32370.8079895363233357No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC30630.7645572906266225No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC29330.7321079116578139No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG28910.721624266144814No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACTAAAAAATAAACCCCTA27610.6891748871760054No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC27520.686928391708934No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT25860.64549303087184No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC17130.4275829705659172No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA15420.3848995566915612No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT15420.3848995566915612No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA15040.37541435360837094No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC14090.3517013459003954No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA13720.3424657534246575No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA13680.34146731099484806No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT13670.34121770038739563No Hit
TCCAACCAATAACACTATCTCTAACCTAAACCTATAAACTCACAAAATCA12890.32174807300611047No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC12490.3117636487080155No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT12360.30851871081113463No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA11070.27631894244977834No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC11030.2753205000199689No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACACCAAACCCCCACAAAAC10820.270078677263469No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC10510.26234074843244537No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC10190.2543532089939694No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC9930.2478633332002077No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT9860.24611605894804106No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA8810.21990694516554177No Hit
ACTCCCACACACACTAACACAATAAGACAACGCTACGAACATCAATACAC8660.21616278605375616No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC8400.2096729102599944No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCACCT7120.17772275250609051No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC6990.17447781460920964No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA6430.16049962059187667No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC6200.15475857662047207No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA6110.1525120811534007No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC5780.14427493110747233No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA5160.12879907344542516No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC5080.12680218858580614No Hit
AAACCTATCAAAACCTCCCCACCAGAAGCCTGCCGCTCTAACCACCCTCG4820.12031231279204442No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCAAAAAATAAACCCCTA4790.11956348096968727No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCG4780.11931387036223493No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC4310.10758217181197333No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACGCAACC4240.1058348975598067No Hit
AAACCTATCAAAACCTCCCCACCCGCAAAACTCCACTCACCCACAAATCT4030.10059307480330684No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)