FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311714
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT132604.253899407790475No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA109203.5032112770039205No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG99793.2013319902218056No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT78092.505181031330001No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA75612.4256209217423663No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT74692.396106687540502No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA74212.3807079566525724No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC68652.2023393238673914No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC63472.036161353035154No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA61361.968471098506965No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA60871.952751560725537No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT53821.726582700809075No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC51391.6486266256889328No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA48701.562329571337829No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC48381.552063750745876No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC45801.469295572223256No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC44221.4186080830504886No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA41301.3249324701489187No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA39391.2636583534906998No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT36211.161641761358168No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC35091.1257113892863329No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA31321.004767190437388No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA31090.9973886318869221No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA28030.8992217224763726No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA26980.8655369986590272No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT26890.8626497366175404No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG26720.8571960194280654No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC26100.8373059920311567No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA24010.7702573512899645No Hit
AAAACCCCACTCTATCTTACAACCTAAATATCCCCTGTAGAACCATGTCG23380.7500465169995573No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC22760.7301564896026486No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC21440.687809979660843No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA21390.6862059451933503No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA17180.5511462430304702No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT16100.5164990985326292No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT16040.5145742571716381No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCA15300.4908345470527471No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC14820.4754358161648178No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT14280.45811224391589733No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA13640.4375806027319915No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC12060.3868931135592242No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA11920.3824018170502448No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA11470.36796550684281104No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA11210.3596245276118493No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA10600.3400553071084392No Hit
TAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCGCCT10320.3310727140904804No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC9320.2989920247406276No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCACCT8910.28583894210718797No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA8770.2813476455982086No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT8280.26562810781678076No Hit
CAACTACCAAACCCTAAAAAACCACTTCATCTAAAAACAAAACTATCTCC8030.2576079354793176No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCACTCTAACCACCCTCG7920.25407905965083377No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT6980.2239232116619722No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACGCCAAACCCCCACAAAAC6430.20627883251955317No Hit
ATCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC5860.1879928395901371No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC5650.18125589482666804No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAGCCCCCACCACCCGCAA5320.17066926734121662No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC5270.169065232873724No Hit
AAACTACAAAATCAAAACCAACCCGAAACCGCACCAAACCCCCACAAAAC4860.15591215024028438No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4810.15430811577279172No Hit
CAAAACAAACAACACTAAAACCAAACTAGTGAGAGGCAGGTCCCGCATCG4780.15334569509229615No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA4560.14628794343532853No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC4390.14083422624585357No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCACC4370.1401926124588565No Hit
ATAATACTCCTTTATCCCAATCCCCGAAACCCTTCACCAAAAATACTAAA4280.13730535041736977No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTAACTCCGCCT4240.13602212284337564No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC4110.1318516332278948No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCGATCCACTC3700.11869855059445517No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAATACCTAAAATAAA3550.11388644719197727No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA3520.11292402651148167No Hit
CATAATCACCCACAACAAATTTCCCACCACCGAAATAAACCACCCCCTAA3340.10714950242850818No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCCAACACC3250.10426224038702143No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)