FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265915
Sequences flagged as poor quality0
Sequence length301
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT109724.126130530432657No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT91693.44809431585281No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG82563.1047515183423275No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA68092.560592670590226No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT64182.4135532030912135No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT63402.3842205215952466No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA62902.3654175206362935No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA59722.2458304345373525No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA54972.067201925427298No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA52301.9667939003064887No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC48251.8144895925389692No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC46331.7422860688565893No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC45181.6990391666509976No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC44541.6749713254235374No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC43901.6509034841960777No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC42311.5911099411466072No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC40601.5268036778669876No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG40471.5219148976176597No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA38261.4388056333790873No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA37461.4087208318447624No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA37261.4011996314611812No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA33681.2665701445950774No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA33091.2443826034635128No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT31441.1823327002989679No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA29791.1202827971344225No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA29661.1153940168850949No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA26781.0070887313615253No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA26190.9849011902299608No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC24200.9100652464133276No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC23040.8664422841885564No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC18480.6949589154429047No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT17090.6426865727770151No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT16640.6257638719139574No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT15130.5689788090179193No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA14260.5362615873493409No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA14160.5325009871575503No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC13490.5073049658725532No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT13310.5005358855273302No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC13080.49188650508621173No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA13010.4892540849519583No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC12300.462553823590245No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA9830.3696669988530169No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA9690.3644021585845101No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC9000.33845401726115487No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT8860.33318917699264805No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA8220.30912133576518813No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT7570.28467743451854916No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA7180.27001109377056576No Hit
AAACTACAAAATCAAAACCAACCCAAAACCGCACCAAACCCCCACAAAAC6830.25684899309929865No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC6530.2455671925239268No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCGATCCACTC6250.2350375119869131No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT6000.22563601150743662No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT5850.21999511121975068No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC5840.21961905120057162No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA5540.20833725062519978No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCA5400.2030724103566929No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC5060.19028636970460486No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA5010.18840606960870954No Hit
TAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCGCCT5000.18803000958953048No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC4860.18276516932102363No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA4680.17599608897580055No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC4660.17524396893744243No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACGCCAAACCCCCGCAAAAC4450.16734670853468214No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCAATCCACTC4300.16170580824699624No Hit
CAAAACAAACAACACTAAAACCAAACCAATAAAAAACAAATCCCGCATCG3900.14666340747983378No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT3890.14628734746065472No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTACAACTCTAAA3880.14591128744147566No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC3670.13801402703871538No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTGAAAAACAATGCC3560.1338773668277457No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA3520.13237312675102947No Hit
AAAAAAAATCCCAAAACAAACATACAAAATCAAAAAAATACCTAAAATAA3430.1289885865784179No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC3380.12710828648252262No Hit
CCAACATATACAAACACAAAATACTCAACGACTACCTACACAAACAAACC3350.12598010642498542No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCACCT3350.12598010642498542No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC3340.12560404640580636No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC3340.12560404640580636No Hit
AAAACCCCACTCTATCTTACAACCTAAATATCCCCTGTAGAACCATGTCG3290.12372374630991106No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA2950.11093770565782299No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACAACCATCAAACA2910.10943346558110675No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)