FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350761
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT150424.288390100381742No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG106183.0271324349058193No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA100612.8683348490852745No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT96942.7637052009773035No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT78042.224876767941704No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA77662.214043180399189No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA75532.1533180712793043No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT70272.0033584121381796No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA68081.9409227365642132No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC66841.9055710298465336No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA64821.8479819592257978No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC64101.8274551617768227No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC57531.6401481350549234No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA56691.6162002046977857No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA49351.4069409084818438No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA47751.3613258030396767No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC43991.2541303052505837No Hit
AAACTACAAAATCAAAACCAACCCCCTAATACCTTGTAGAACCATGTCGT43921.252134644387489No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC42421.2093704830354572No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA40561.156342922958938No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA40051.141803108099247No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA39731.1326800870108136No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC35671.0169317569513143No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT35641.0160764737242738No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT35601.0149360960882197No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG34090.9718868403271743No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC33310.9496494764241179No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA31250.8909200281673276No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT31060.8855032343960703No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC29800.8495813388603637No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA28660.8170805762328195No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC26960.7686145267005169No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA24060.6859371480865889No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC22660.6460239308246926No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC22160.6317692103740153No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA20640.5884348602039565No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA17690.5043320095449608No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA17300.49321332759343256No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA16380.4669846419641865No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT16270.4638486034650374No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA15060.4293521799743985No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA14690.4188036868408973No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC14480.4128167042516129No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT13830.39428556766573253No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCACCT13630.38858367948546163No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC13210.3766097143068927No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCG13000.3706227317176083No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC12690.3617848050381884No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC12310.3509512174956737No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACGCCAAACCCCCGCAAAAC11920.33983253554414544No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT11090.3161696995960212No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT11050.315029321959967No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC10570.30134479032731687No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC9880.2816732761053823No Hit
CAACCATAAATACACAAAAAACAACCCTTCTTGTAGAACCATGTCGTCAG9730.2773968599701791No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA8740.24917251347783817No Hit
TAAAACTAAACCCCACATACCTTCCCCAACCCAAACACAACACTAACACA7790.22208854462155142No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCAAAACCCAAAAAA7380.2103996738519961No Hit
CACCCAAAATAAACTATCCCCATCACACAATGTAGAACCATGTCGTCAGT7270.2072636353528471No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA6860.19557476458329173No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA6620.18873249876696668No Hit
AAAACAAAAAAAACTCCTAATCCCTACAACCTCAAAACGACCATCAAACA6570.18730702672189897No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA6430.18331570499570934No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC6340.18074985531458743No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA6140.17504796713431653No Hit
CCCAAAAATTAATAAATAACCACTCACCTACTTAATCTCCCTGTAGAACC5780.1647845684098289No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA5760.16421437959180182No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC5460.15566154732139548No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC5360.15281060323126003No Hit
CCCAAAAATTAATAAATAAACCACTCACCTACTTAATCTCCCTGTAGAAC5090.14511305418789433No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC4890.13941116600762343No Hit
AAACTACAAAATCAAAACCAACCCCAAAACTAAAATAACCCCAAAACAAC4780.13627512750847445No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACGAACATTAAAACCTTCATC4650.13256890019129836No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC4610.13142852255524418No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTGCCTCCGCCT4580.13057323932820353No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA4460.127152106420041No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA4390.1251564455569462No Hit
CATAATCACCCACAACAAATTTCCCACCGCCAAAATAAACCACCCCCTAA4200.11973965178568884No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC3720.10605512015303868No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATGATCACA3650.10405945928994387No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)