FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382601
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT155144.05487701286719No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA113112.9563435537282965No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG111002.9011947172119257No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT104342.7271230341792103No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA91402.3889116860645947No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA82012.1434862951220723No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT78292.0462570667614566No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA75421.9712441943434542No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT70781.8499690277861272No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC70331.838207427581214No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA67291.758751283974689No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC58361.5253488621305222No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC58121.519076008687902No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA56511.4769956168436569No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC54541.4255059448354814No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT53091.3876074552863165No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC52301.3669593127043578No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA47731.247513728401128No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC46601.2179790434421238No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC44321.1583869357372303No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA43581.139045637622484No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA43221.1296363574585533No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG40601.0611577073766143No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA39841.0412936714749832No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT38611.0091452975815536No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA33920.8865632865570137No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA33670.8800290642209508No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA32270.8434374191389985No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC30360.793515960491478No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA29480.7705154978685367No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC28770.7519583064341181No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC28470.7441172396308425No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA26080.6816500740980813No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT24760.6471493801636692No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA20370.5324084359424047No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA18680.48823709295061957No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT16020.41871296729491037No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA13820.36121181073755687No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT13430.35101842389329874No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC12920.33768861032773045No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC12900.3371658725408454No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC12410.3243587967621621No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT11390.2976991696310255No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC10450.27313049364742903No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA9780.2556187777867805No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA9710.2537891955326829No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT9560.24986866213104514No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACGCCAAACCCCCGCAAAAC9490.24803907987694754No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC9430.2464708665162924No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCGCAAAAC9300.2430730709015397No Hit
AAAACCCCACTCTATCTTACAACCTCTCTATCCAACACCCTAAACCCACA9100.23784569303268943No Hit
CAACCATAAATACACAAAAAACAACCCTTCTTGTAGAACCATGTCGTCAG8580.2242545105736786No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCGCACCAACAT8190.2140611237294205No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC8060.21066332811466776No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCGAACTCATTCCACACCAACAT7990.20883374586057016No Hit
TAAAACTAAACCCCACATACCTTCCCCAACCCAAACACAACACTAACACA7940.20752690139335758No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACAACCATCAAACA7870.20569731913925993No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCACTCTAACCACCCTCG7700.2012540479507372No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA6980.18243548762287606No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC6540.17093525631140535No Hit
ATAATACTCCTTTATCCCAATCCCCAAAATCCTTCACCAAAAATACTAAA6320.16518514065567003No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACGCCAAACCCCCGCAAAAC6270.16387829618845742No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC6230.16283282061468737No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC6120.1599577627868197No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC6100.15943502499993464No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACACTAAAACCTTCATC5780.15107122040977417No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA5680.14845753147534899No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA5230.13669593127043578No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCGATCCACTC5210.13617319348355075No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT5130.13408224233601063No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA5070.13251402897535552No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATGCC5000.1306844467212579No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA4980.1301617089343729No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCAAAAAATAAACCCCTA4870.12728665110650522No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC4540.11866147762290219No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCA4400.11500231311470696No Hit
CCAAAACTCAACAACCTAAACCCTGTAGAACCATGTCGTCAGTGTAGATC4380.11447957532782194No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCACCT4310.11264999307372434No Hit
CATAATCACCCACAACAAATTTCCCGCCGCCAAAATAAACCGCCCCCTAA4300.11238862418028181No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC4200.10977493524585666No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT4030.10533166405733388No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA3910.10219523733602369No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)