FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334433
Sequences flagged as poor quality0
Sequence length301
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTCGTTTGAGGTTTTTTGT151744.537231672711724No Hit
TTAGGGTGGAAGATAGAGATTTGTGTTTATAGGGTTTAGGAAGGCGGGTA129463.871029473766046No Hit
GATTGTTTAGGGAGGGGGTTAATAGCGTGTTAGTATTGAGGTTAGGTTTG92752.773350715987956No Hit
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTCGGAAGTATGGTATTT91282.7293957235081465No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTTCGTTTATC88512.6465689689713634No Hit
GTTGGGGATTGAGGTTTTTAGAGTAGAGGAGTTAGTTGTGGGTATTAGTG78542.348452455349801No Hit
GTTAAAAGAATTTGGAAGGGTTTGTGAGATATTGGATTGCGGGGTAATAA71762.145721265544967No Hit
GGTGGGGAATAAGGTTTTGTATTTATTGAGTTTTTGTTATAGGTTTGGGT69892.089805730893781No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGTGGTGTTGAT64531.9295344657973346No Hit
TGATTTTGTGAGTTTATAGGTTTAGGTTTTAGTAGTAGAATTATTTTGGT62481.8682366871690295No Hit
GGTTGTAGGAGGTTTTTAGTAAGGGAGGGGTTGTAGGAGTGTTTTTGGGG61271.832056047100615No Hit
TGGTTTTTATTTTTGTGTTTTTTGGGGATGGTTTTTAAGGATAGTATGTT59031.7650770109409057No Hit
GTGAAGTTTTTGGTTTTTTGTTGTATAGATGAGGTTGGGATGTAAGGAGA56851.6998920561069033No Hit
AGAAGGGTTAGTTATAGGGGAGGATGTGGTATAGGTGTTTATGGGATTTT55961.6732798497755903No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGT54981.6439765214557176No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTCGTGTAGAGGGTTCGTTGGGGTTT53871.6107860169301473No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGCGTCGGGTA51811.5491892247475578No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTT43391.2974198120400797No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTTCGTTATTTTACGATGAGTTAG43041.2869543376401253No Hit
TGGGATATTTTTGATTTATATTTGGGTTTTAGGGAGTTAAGGTATTGTGG41931.253763833114555No Hit
GAATTGAAGGAGAATTTATTTGTGGTTGTTATCGTTTTATTAGGATAGTT36721.0979777713323746No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTTGTTTGAGGTTTTTTGT35951.0749537276524743No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTCGGTGATTGTTTCGGAGGAGAGG35761.0692724701210707No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTTATGGGATAGGGAGTAT34561.0333908436069408No Hit
GATAGGTTTGGAGATAGTTTTGTTTAAAGATATTTAGTATTTTAGTGGTA31640.9460788857558914No Hit
GGAAAGTTAGGTAGTGAGTTGTTTGATATTTGTAGTATTTATGTTGGAGT31230.9338193300302302No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTTGTGTAGAGGGTTCGTTGGGGTTT30540.9131873947846055No Hit
GTTGGTTTTTTTGGGGTGAAGCGGTTTTTATTTTCGTTGATGAACGGTTT27740.8294635995849693No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGTGTTATTTTGTGATGGGTTAG25820.7720529971623613No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTAGTATTTTTGATTTTTTTGATT24960.7463378314939016No Hit
TGTTGGGTAGGTATTGTTAGTTTTGTTGTATGGATGAAGGCGTGAAGGTT24000.7176325302825977No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTTAGTTTTATTTTTAAATT22970.6868341341913029No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGTGGGGGGTAT22430.6706874022599445No Hit
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTCGGAAGCATGGCACTT19650.5875616341688769No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGCGGGGGGTAT19180.5735079971175093No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTTGTGGCGGGGGGTAT18090.540915519700508No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGCGGTGTTGAT17560.525067801323434No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTGTTT17350.5187885166834613No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGACGTTGAGCGTGAAGAAGTGG16740.5005486898721119No Hit
GATAGGTTTGGAGATAGTTTTGTTTTTATGGGGTTTTTTTTGGGTTATTT16630.49725954077498336No Hit
GGGGTGTATTTATTAGTTTTAGGGATGTATTTTTAGGTTTCGGGGGTTTA16140.48260787661504695No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTTGGGGTAAGTGTTTATTTTTTTT15090.4512114534151833No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTG14400.4305795181695586No Hit
GGAGTTGAGTGGTATAGTTGGTATTATTACGTATTAGTAGAGGTTTTTTG14120.42220713864959497No Hit
TTGTGTGATGGGGATAGTTTATTTTTTATTTTTTGAGTTTTTATATTTAC13290.39738901364398843No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGTGTGAAGAAGTGG12960.38752156635260276No Hit
GAATTGAAGGAGAATTTATTTGTGGTTGTTATTGTTTTATTAGGATAGTT12920.3863255121354651No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGCGTTGGGTA12250.36629160399840927No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTAATTAATTGAGAAGTTGT11830.3537330347184638No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTAATTAATTGAGAAGTTGTT11110.3322040588099858No Hit
AATTTGTTGTGGGTGATTATGGTGTATGGAGTTAGTGTGGATTTGTGTGG10320.30858198802151704No Hit
GGAAAGTTAGGTAGTGAGTTGTTTGATATTTGTAGTATTTACGTTGGAGT9810.2933322967530118No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTTGTGTAGAGGGTTTGTTGGGGTTT9800.2930332831987274No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTATGGGATAGGGAGTATT9780.2924352560901586No Hit
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTTGGAAGTATGGTATTT9140.27329838861595596No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTTTT8880.26552403620456116No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGTTT8850.2646269955417079No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGCGTGAAGAAGTGG8760.2619358735531482No Hit
TTTAAGGTTTGTGTTGATGTTAATGTTATAGGTTACGATAAGGTATGTTA8710.26044080578172607No Hit
GTGAAGTTTTTGGTTTTTTGTTGTATAGACGAGGTTGGGATGTAAGGAGA8260.24698519583892736No Hit
TTTGGAGAGGAGGTTTTTAGATTTTATTTTGGTGGTATAGTGTTTTTTTT8110.24249999252466112No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTT7930.23711774854754167No Hit
TTTTTAGGTGAGATTAGGGATTTGGGCGTGGGGGGCGGTTAAAGGTATTT7930.23711774854754167No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTCGGTGATTGTTTTGGAGGAGAGG7790.23293155878755983No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGTGTTATTTTGTGATGGGTTAG7580.22665227414758712No Hit
GTTGGTTTTTTTGGGGTGAAGCGGTTTTTATTTTCGTTGATGAATGGTTT7540.22545621993044945No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTAGTTTTATTTTTAAATTT7230.21618679974763255No Hit
TAGGTTTTATTGTGAGGATGATTTGGGTGATACGTTGTTGGTTGTTTTGA6920.20691737956481568No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTAATTAATTGAGAAGTTGTTT6740.2015351355876962No Hit
AGGTATTAGGGAGTTGTATGGGGTACACTGACGACATGGTTCTACAAGGT6380.19077064763345722No Hit
TGTTGGGTAGGTATTGTTAGTTTTGTTGTATGGATGAAGGTGTGAAGGTT6150.18389333588491566No Hit
AGGTATTAGGGAGTTGTATGGGGTTGGTTTTGATTTTGTAGTTTAGACCA5840.17462391570209876No Hit
GGAGTTGAGTGGTATAGTTGGTATTATTATGTATTAGTAGAGGTTTTTTG5810.17372687503924553No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTTAATTAATTGAGAAGTTG5660.16924167172497928No Hit
GGGTTTAGGTTGTTGAGTTTTGGGGATTGGGATAAAGGAGTATTATAGAC5600.16744759039927282No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTCGTTTATCG5580.16684956329070397No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTATTTCGTGATGGGTTAG5200.15548704822789616No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGAGGCGTCGGGTA4720.14113439762224422No Hit
GGGGATATTTAGGTTTGTTTTTTTTTGGGTTTGGTGTTTTTGGAGTTTTG4620.13814426207940006No Hit
GAATTTTGGGTTTGATTTTTGTTTCGGTTCGGGGTGTAGGCGGTGTTGAT4600.13754623497083124No Hit
TGGGTTGTGATTAGATGGTAGAAAGGGGTTTTAGTTAGGTTGATAATGGT4520.13515412653655592No Hit
TGATTTTGTGAGTTTATAGGTTTAGGTTTTAGTGGTAGAATTATTTTGGT4320.1291738554508676No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTATTTCGCGATGGGTTAG4270.12767878767944552No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTGTTTTT3820.1142231777366468No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTGTTTT3500.10465474399954548No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTCGGTGATTGTTTCGGACGAGAGG3430.10256164911955458No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)