FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334433
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT132143.9511651063142694No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG121033.6189610475042837No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT102523.06548695852383No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT93792.804448125633535No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA87732.623245911737179No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA77872.328418547212745No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA67582.0207335998540814No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT65651.9630239838771892No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA63411.8960449477174801No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC59111.7674691193751813No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA57981.7336805877410422No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC56711.6957058663469213No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC54591.6323149928386256No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA52861.5805856479474214No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG50741.5171947744391254No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC50681.5154006931134187No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC44451.329115248794228No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA44201.3216399099371174No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC41441.2391121689546187No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA41061.227749653891811No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA40301.2050246237661952No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT39331.1760203090006072No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA39231.1730301734577628No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC38871.1622656855035238No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA35231.0534247517439965No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA33010.9870437426928563No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA32200.9628236447958186No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC31110.9302311673788174No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA30660.9167755574360186No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA28180.8426201959734835No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT25350.7579993601109938No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC24760.7403575604082133No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA23630.7065690287740743No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA22580.6751726055742107No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC22570.6748735920199262No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC21350.6383939383972276No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT19500.5830764308546107No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCGCAAAAC18710.5594543600661418No Hit
CAACTACCAAACCCTAAAAAACCACCAACCACAACCGAAACCCTCCTCCC16510.4936713781235703No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAGAACCTCAACAAA16240.4855980121578911No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC15570.46556410402083526No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACAACCATCAAACA14250.4260943148552924No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT13680.4090505422610807No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA13670.4087515287067963No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA12700.379747213941208No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT12140.3630024549012807No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA12080.36120837357557417No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC12060.36061034646700535No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA11640.3480517771870599No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC10750.32143957085574687No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT10710.3202435166386092No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC10640.31815042175861835No Hit
AAAACCCCACTCTATCTTACAACCTAAATATCCCCTGTAGAACCATGTCG10570.31605732687862736No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC10480.31336620489006767No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC9430.28196978169020404No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC9230.2759895106045157No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT8200.2451911145132209No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCACCT7860.23502465366755076No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACAACCTTAAAAAACAATACC7410.22156904372475206No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCGATCCACTC6550.1958538780562923No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAATACCTAAAATAAA6250.18688347142775982No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC5920.1770160241363741No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACTCAACAACCTAAACCCTGTA5550.16595252262785073No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCACC5380.16086929220501567No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT5340.159673237987878No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT5160.15429099401075852No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC5100.152496912685052No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT4820.14412453316508836No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC4700.14053637051367537No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4670.13963932985082214No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA4060.12139950303947278No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA3900.11661528617092212No Hit
ACTCCCTTTTCCCTTTAACTTTATTATACGTCTCTCTAAAAATTTCTAAA3830.11452219129093122No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCAAAACCCAAAAAA3360.10046855423956368No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)