FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396655
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT176864.458786602967314No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA123103.1034526225561256No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT111372.8077296391070328No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA111202.8034437987671903No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG107102.700079414100415No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT106752.691255625165446No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC102742.5901602147962337No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA96492.4325925552432213No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT90452.2803191690511904No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC69621.7551776732929119No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC68121.7173614350001891No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC67641.7052602387465179No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG62081.5650880488081582No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT61901.5605501002130313No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA61421.54844890395936No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC60201.5176916968146124No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA54771.3807969141949552No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC53701.3538213308794795No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA50731.2789451790598882No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC50621.2761719882517553No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA46611.1750765778825427No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA44131.1125537305719075No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA42121.061879971259659No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA38960.982213762589656No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA37200.9378427096595279No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT34940.8808662439651586No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC33370.841285247885442No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC33040.8329656754610429No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA30400.7664090960658507No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC30110.7590979566625908No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA27200.6857344543747085No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA26980.6801880727584425No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA26390.6653136856966381No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA22280.5616971927745774No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT20860.5258978205241331No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT18750.4727029786590362No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA18130.4570722668313774No Hit
AAACTACAAAATCAAAACCAACCCAAAACCGCACCAGACCCCCGCAAAAC17370.4379120394297311No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT17070.43034879177118657No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCACTCTAACCACCCTCG17050.42984457526061687No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAGACCCCCACAAAAC16100.40589429100855906No Hit
AAACCTATCAAAACCTCCCCACCAAAAGCCTACCGCTCTAACCACCCTCG15850.39959158462643857No Hit
CATAATCACCCACAACAAATTTCCCGCCACCAAAATAAACCACCCCCTAA15690.39555785254188147No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA15580.39278466173374843No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA15370.3874903883727672No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCGACAT13890.350178366590614No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT13470.3395898198686516No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC13300.33530397952880964No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCAAAAAATAAACCCCTA12860.32421121629627764No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC12640.31866483468001155No Hit
TAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCGCCT11530.29068081834339665No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC11360.28639497800355473No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC11030.2780754055791557No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC10760.2712684826864656No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC10630.2679910753677629No Hit
CAAAACAAACAACACTAAAACCAAACTAATGAGAGGCAGGTCCCGCATCG10390.2619404772409272No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA9470.23874651775472386No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA9340.23546911043602123No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCCAAAATAA8960.2258889967351981No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA8320.20975406839696967No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC7770.1958881143563046No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT7600.19160227401646268No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCGATCCACTC7280.18353480984734843No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT6900.1739546961465253No Hit
CACCCAAAATAAACTATCCCCATCACACAATGTAGAACCATGTCGTCAGT6730.1696688558066834No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC6170.1555507935107335No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT5390.1358863495985176No Hit
ATACAAAACAAACCCCTATCTCCTCTACTACCTTCTATTCTAAATCTCCA4720.11899509649443471No Hit
AAACCTATCAAAACCTCCCCACAAATCTCTATCTTCCACCCTAATGTAGA4650.11723033870744098No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC4570.11521347266516242No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACTCTTCACCAAAAATACTAAA4570.11521347266516242No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA4330.10916287453832675No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA4310.10865865802775712No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA4310.10865865802775712No Hit
CCTAAAAAAACAACACTCCAAACACGTATACCCGAACCCTAAATCCCACC4070.10260805990092146No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)