FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences313275
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT130274.158327348176522No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG98073.1304764184821643No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA93322.9788524459340837No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA76812.451839438193281No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT67012.1390152421993456No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA63702.033357273960578No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA62651.9998403958183704No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA61541.9644082674966084No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT61531.9640890591333493No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA60891.9436597238847657No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC55701.7779905833532839No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT53761.7160641608810152No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC53391.7042534514404277No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT53101.6949964089059133No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC51981.6592450722208922No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA50831.6225361104460938No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC49911.5931689410262548No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC49441.5781661479530764No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC48511.5484797701699784No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA47161.5053866411299976No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG45711.4591014284574255No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA40861.3042853722767538No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA37291.1903279865932488No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC36641.1695794429814061No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC36321.1593647753571144No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA35611.136700981565717No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA33491.069028808554784No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA33171.0588141409304923No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA32321.0316814300534674No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC27530.8787806240523501No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCACCT26550.8474982044529566No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC23930.7638656132790679No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC23690.756204612560849No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTAGCTCCGCCT23050.7357752773122656No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC18840.6013885563801772No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA16460.5254169659245073No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA16010.5110525895778469No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA14920.4762588779826032No Hit
ATACAAAACAAACCCCTATCTCCTCTGCCGCGACCTTAAAAAACAATACC14260.4551911260075014No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT12990.41465166387359353No Hit
CTCATTCAAAAAACCCAAAAAACCGAGCCCAGCTCGTTCCGCGCCAGCGT12770.4076290798818929No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT12070.3852844944537547No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT11760.37538903519272204No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA11700.37347378501316736No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA11110.3546404915808794No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC10730.342510573777033No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT10710.3418721570505147No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT10090.32208123852844944No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAGACCCCCACAAAAC10040.32048519671215386No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC9750.3112281541776395No Hit
TAAAACTAAACCCCACATACCTTCCCCAACCCAAACACAACACTAACACA8990.28696831856994653No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC8930.28505306839039185No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATGCC8890.2837762349373554No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC8790.2805841513047642No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT7570.24164073098715189No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCACCTATCCTATAA7480.2387678557178198No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC6870.21929614555901367No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA6550.2090814779347219No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA6050.19312105977176602No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAAATCCTATAACTCTAA5420.17301093288644162No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAACAACATT5360.1710956827068869No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC5140.16407309871518636No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA4860.15513526454393106No Hit
ACTCTAAAAAACCAACTCCTCCAAACAGAAACTCCTAAAACCTCAACAAA4420.1410900965605299No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC4300.13725959620142048No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC4280.13662117947490224No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCAAAAAATAAACCCCTA4240.13534434602186576No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCACAACCACGCTACCTT4120.13151384566275637No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC4070.1299178038464608No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT3580.11427659404676402No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT3510.1120421355039502No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC3490.11140371877743198No Hit
CTCATTCAAAAAACCCAAAAAACCAAGCCCAACTCATTCCACACCAACAT3260.10406192642247226No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)