FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370732
Sequences flagged as poor quality0
Sequence length301
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT173874.689910771123076No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG112313.0294120820430934No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA105942.8575898492711715No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT96562.604576891123507No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT89172.4052415221777457No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA87932.3717941801624893No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA83092.2412416516513276No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT79232.1371233127973848No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA77352.0864128265161894No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA77132.080478620674773No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC70211.8938208732993105No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG65441.7651565011922359No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC64651.7438473074889678No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC59771.6122158324611848No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC56921.5353408931519266No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC53221.4355383403644681No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC49661.3395121003851838No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA47021.2683016302881867No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA41941.1312754226772979No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA41051.1072688626824767No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT40761.0994465004369733No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA39131.0554794298846606No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC38511.0387557588770324No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC38021.0255386640484232No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA37191.0031505238285339No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA37030.9988347377620492No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA35500.9575650335012894No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA34560.9322097903606918No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC33740.9100913867699577No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA32000.8631572132969368No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT30070.8110980438699653No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC26630.7183086434405447No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT25950.6999665526579848No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAAGTCCTAAAACCTCAACAAA22940.6187758272822417No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT21200.5718416538092207No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA19570.527874583256908No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC18990.512229858765901No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA18690.5041377598912422No Hit
TAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCGCCT15640.42186808799887787No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC15230.4108088862035109No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA15030.40541415362040506No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA13700.3695391819427511No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC13440.36252602958471347No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA13220.356591823743297No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC12690.3422957823980665No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA12140.3274602677945254No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC11860.31990764217817724No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT11830.31909843229071133No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCAAAAAATAAACCCCTA11400.30749975723703377No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC11320.3053418642037914No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCAATCCACTC11180.3015655513956173No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCACTCTAACCACCCTCG11040.2977892385874432No Hit
CAACCATAAATACACAAAAAACAACCCTTCTTGTACATCCTCCCCTATAA8860.2389866534315894No Hit
TACTATCCACACTCTCTTACCTCCCGACCCCCACACTCCCTTCTAACACC8730.23548007725257059No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT8510.22954587141115415No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCGATCCACTC8280.22334192894058244No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA7820.21093404399943894No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT7590.20473010152886723No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA7590.20473010152886723No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACACACACTTCAAT6760.18234196130897792No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA6760.18234196130897792No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC6580.17748670198418265No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACGCCAAACCCCCACAAAAC6530.17613801883840619No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC6430.17344065254685326No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCACC6260.16885512985121326No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC6250.16858539322205798No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA6240.16831565659290268No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC6210.1675064467054368No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT6010.16211171412233097No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTACTTAAAATTCAAAACCTTATT5150.13891436401497578No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC5000.1348683145776464No Hit
AATACCTAAAAACCCAAAACCCTTCTAAATAAAAAACCCTATAAAACTTC4740.12785516221960877No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCGATTCACCCA4560.1229999028948135No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACACTACCTT4470.12057227323241587No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA4360.11760517031170765No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT4270.11517754064931No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC3940.10627623188718535No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC3870.1043880754830983No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCACTTACCAATCCACTC3730.10061176267492421No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)