FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329078
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT142724.336965704179556No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG117783.579090671512529No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT91762.7883966719136497No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA89042.7057414959371333No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA84632.5717307142987376No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA70812.151769489300409No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT68722.088258710700807No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA62391.8959030989613403No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC57471.746394471827348No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC56271.7099289530141792No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC55821.6962543834592405No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT52611.5987091206340138No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC51631.5689289469365926No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC48611.4771573912567841No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA48291.4674332529066056No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC45761.390551784075508No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA43671.3270410054759054No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT42731.2984763490722564No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC40711.2370927257367554No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA39751.2079203106862204No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA35901.0909267711606365No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC35171.0687435805492922No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA34401.0453448726441756No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA33791.026808233914148No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA31380.9535733169643672No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA31370.953269437640924No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG31300.9511422823768225No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA31110.945368575231404No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA29570.8985711594211706No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT29000.8812500379849155No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA26620.8089267590054637No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC24950.7581789119904704No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC24120.7329569281446952No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT22580.6861595123344617No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA19470.5916530427436656No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA18910.5746358006308534No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC17600.5348276092598107No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT17430.5296616607612784No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT14340.4357629498173686No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA14120.42907760470162093No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC13850.4208728629686579No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC13750.41783406973422715No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACAACCATCAAACA13000.3950431204759966No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA12720.3865344994195905No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC12080.36708622271923375No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA10900.33122846255295096No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCG10530.3199849275855572No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC10230.310868547882265No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC8960.2722758738049945No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT8550.2598168215438285No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT8550.2598168215438285No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACACACACTTCAAT7990.24279957943101638No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT7370.22395906137754576No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC7210.21909699220245657No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT5810.17655388692042617No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCGACTCATTCCACACCAACAT5730.17412285233288158No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA5640.1713879384218939No Hit
AAACTACAAAATCAAAACCAACCCAAAACCGCGCCAAACCCCCGCAAAAC5280.1604482827779432No Hit
TAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCGCCT4860.1476853511933341No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTGCGGACATCAATACAC4860.1476853511933341No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC4770.14495043728234644No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA4750.14434267863546027No Hit
AATACCTAAAAACCCAAAACCCTTCTAAATAAAAAACCCTATAAAACTTC4610.14008836810725725No Hit
ATAATACTCCTTTATCCCAATCCCCAAACCCCTACCAAAACTCAACAACC4500.13674569554938343No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA4430.1346185402852819No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA4310.13097198840396504No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCCA4130.12550216058198968No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC4120.12519828125854662No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACGAACATTAAAACCTTCATC4080.12398276396477431No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC4040.12276724667100201No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA3970.1206400914069005No Hit
CAACTACCAAACCCTAAAAAACCACACACATTCAAAACAACACCCCATAC3820.11608190155525439No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC3300.10028017673621452No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)