FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000888489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443929
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT214374.828925346170221No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG158963.5807527780343253No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA131452.9610590882776298No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC122982.770262812296561No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT116372.6213651282074384No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA94342.1251146016592743No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT85021.915171119706079No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA83641.8840850676572156No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC80871.8216877023127571No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC77781.7520819770729101No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC76271.718067528816545No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT75671.7045518540126912No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC67191.5135303167848912No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA62911.4171185031840676No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA58651.321157212076706No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA58311.3134983296878555No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT57951.3053889248055432No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA56411.2706986928089852No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA55471.249524135616281No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC54051.2175370385804938No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA54031.2170865160870319No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC53831.212581291152414No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA50441.13621772851064No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA44140.9943031430701756No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC43010.9688486221895843No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA42760.9632170910213119No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA42560.958711866086694No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA41300.9303289489986011No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA39380.8870787896262691No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT36850.8300876942033523No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC36810.8291866492164287No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCG34600.7794039136889007No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT31280.7046171797742431No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA28430.6404177244559378No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC27090.6102327173939978No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA25960.5847781965134065No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA25850.5823003227993666No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC25270.5692351704889745No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT24640.5550437119449281No Hit
TAAAACTAAACCCCACATACCTTCCCCAACCCAAACACAACACTAACACA23860.5374733346999182No Hit
AAAAAAAATCCCAAAACAAACATACAAAATCAAAAAAATACCTAAAATAA23750.5349954609858784No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA21900.4933221303406626No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG20860.4698949606806494No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA19030.4286721525288954No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC18810.42371640510081565No Hit
CATAACACAAACACAAAAAACCAACTATCCTAATAAAACGATAACAACCA17830.40164080292118787No Hit
AAAACAAAAAAAACTCCTAATCCCTACAACCTCAAAACGACCATCAAACA17670.3980366229734935No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCGCAAAAC17420.3924050918052211No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA16400.3694284446386697No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC16360.36852739965174613No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT15780.3554622473413541No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA15680.35320963487404516No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACACACACTTCAAT15670.3529843736273143No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT13710.3088331692680586No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA12840.28923544080247066No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC12590.28360390963419824No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAGGGGGAAGCAACAT12250.2759450272453478No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT11880.26761036111630465No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC11840.26670931612938104No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC11430.2574736050134143No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC10670.24035375026186617No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAACAAACCACCCCCTAA10190.22954121041878317No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC7380.16624280008740136No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA7360.16579227759393958No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTGA7220.16263862013970704No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA7190.16196283639951434No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCTTAAAACCTCAACAAA7120.16038600767239805No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA7050.1588091789452818No Hit
ATACAAAACAAACCCCTATCTCCTCCTTTCAACCAAAAACCTCCACAAAA6840.15407869276393296No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACAACCATCAAACA6140.13831040549277024No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT6030.1358325317787304No Hit
CCAACATATACAAACACAAAATACTCAACGACTACCTACACAAACAAACC5980.1347062255450759No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC5430.12231685697487661No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCGCC4960.1117295783785245No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAGAAAAATAATCACA4820.10857592092429194No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA4640.10452121848313581No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)