FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000888491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436563
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT228705.238648259243225No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA169503.882601136605713No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG161613.7018712075920313No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT147973.389430620551902No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT136013.115472451856891No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT127462.919624429921913No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA114582.6245925559426704No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA109412.5061674947258474No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC103582.3726243405877274No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC97612.2358743182541807No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC83211.9060250181531644No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC74861.714758236497367No Hit
AAAAATCCTAAAAACCCCAAAACCTACCTAATGTAGAACCATGTCGTCAG73081.673985198012658No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA72561.662073973286788No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA69601.5942716171549125No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA69481.591522872987404No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC69241.586025384652387No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA68451.5679294855496229No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC59001.3514658823583308No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACGAACATTAAAACCTTCATC52921.2121961778712351No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA51191.1725684494563213No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC50841.164551278967755No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT49901.1430194496556052No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA43370.9934419545403528No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT38000.8704356530443488No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT36210.8294335525456806No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC32070.7346018787666384No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA31250.7158187936219973No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA28510.6530558017972206No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT24480.5607438101717278No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC24130.5527266396831614No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA23840.5460838412783493No Hit
CTCATTCAAAAAACCCAAAAAACCCAACTTTACTACTCCCTTTTCCCTTT23710.5431060350968818No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC22530.5160767174497152No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACACTAAAACCTTCATC21400.49019270987234376No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACAAACATCAATACAC19910.4560624697924469No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC19330.4427768729828227No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC17370.397880718246851No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT16820.38528230747910386No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT16580.37978481914408685No Hit
AATACCTTAACTCCCTAAAACCCAATGTAGAACCATGTCGTCAGTCTAAA15340.351381129413166No Hit
CCCAAAAATTAATAAATAAACCACTCACCTACTTAATCTCCCTGTAGAAC14560.333514292324361No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT14490.33191085822664773No Hit
CAACTACCAAACCCTAAAAAACCACGTCTCAACTACCAAACAAAACTATC14380.32939117607309826No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA13540.310149966900539No Hit
ATACAAAACAAACCCCTATCTCCTCCCTATAATCTAACCATCAACCCCGA10510.2407441766709501No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA9230.2114242388841931No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA8860.20294894436770866No Hit
AAAAATCCTAAAAACCCCAAAACACAAATTCCAAAATCTACCTAATGTAG8490.19447364985122423No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT7680.17591962672054207No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT7050.1614887198411226No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC6550.15003561914317062No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAATCGACCATCAAACA5260.12048661934245458No Hit
AATACCTTAACTCCCTAAAACCCAATGTAGAACCATGTCGTCAGTGTAGA5100.11682162711910997No Hit
CAACTACCAAACCCTAAAAAACCACAAACACTCAACACCAACCCATAAAA4870.11155320079805206No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAGACATTAAAACCTTCATC4820.11040789072825687No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)