FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000888493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407103
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT168614.141703696607492No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA158043.882064244183904No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG123073.023067872258372No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA116602.8641400333576517No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT110662.718231012790375No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC94932.331842310177031No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA89512.1987064698614356No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA89042.1871614800185704No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC83722.0564820205206056No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT78151.919661608978563No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT76971.8906763153305184No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT67501.6580570519008704No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC65701.6138421971835137No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC58301.4320700166788258No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA57731.4180686460183296No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC56741.3937504759237833No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA55001.3510094496970053No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC54151.3301302127471426No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC53291.3090053377155166No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA51731.2706857969604743No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA45981.1294438999466967No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA38660.9496368240961133No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC37450.9199146162027793No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT33490.8226419358245948No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC30780.7560740156667969No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA29710.7297907409181461No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA28880.7094027801318094No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA27700.6804174864837645No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA26670.6551167640621661No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG25300.6214643468606225No Hit
TAAAACTAAACCCCACATACCTTCCCCAACCCAAACACAACACTAACACA24560.6032871288101537No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC23090.5671783307909792No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA22200.545316541514064No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC21900.5379473990611713No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCAAAAAATAAACCCCTA19900.48881978270855286No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA19830.48710031613621124No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA19520.4794855356015554No Hit
CCCAAAAATTAATAAATAAACCCCAAAAATTAATAAATAAACCCCAAAAA19460.47801170711097685No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT17380.4269189861042537No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA17260.42397132912309665No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT16900.4151283581796253No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT16450.40407464450028613No Hit
AAAACAAAAAAAACTCCTAATCCCTCCTACGAATTTCTATATTCAAATAT14410.3539644758206154No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCG14180.3483147999400643No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC13500.33161141038017405No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA13040.3203120586190718No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA12900.31687312547438856No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATCCCACACCAACAT12220.3001697359144983No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC11910.29255495537984244No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT11630.28567708909047584No Hit
AAAAAAATCCTTCCAACCAATAAAACTAACAATACCTACCCAACATGTAG11270.2768341181470046No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACACACACTTCAAT10960.26921933761234873No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC10870.2670085948764809No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC10770.26455221405884993No Hit
CCCAAAAATTAATAAATAAACCCCAAAAATTAATAAATAAACCACTCACC10570.25963945242358816No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC10510.2581656239330096No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC10400.2554636050336156No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA10360.25448105270656324No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA9180.22549575905851837No Hit
TACTATCCACACTCTCTTACCTCCCGACCCCCACACTCCCTTCTAACACC8300.20387960786336629No Hit
ACTCCCACACACACTAACACAATAAAACAACACTACGAACATCAATACAC8120.1994581223916306No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA8070.19822993198281516No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA7960.19552791308342113No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA7580.18619366597642364No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCGCAAAAC7510.18447419940408202No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACTCCAACAT6710.16482315286303464No Hit
CCAACATATACAAACACAAAATACTCAACGACTACCTACGCAAACAAACC6540.1606473054730621No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT6290.1545063534289848No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA6220.15278688685664316No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT6190.1520499726113539No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCAATCCACTC6180.1518043345295908No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAACAACATT5950.14615465864903968No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT5780.1419788112590671No Hit
CAACTACCAAACCCTAAAAAACCACTCTACACTAACCGAAACCCAAAAAA5630.13829424003262072No Hit
CCTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA5620.13804860195085764No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCAATCCACTC5240.12871435484386015No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCACTTACCAATCCACTC4990.12257340279978285No Hit
CAACCATAAATACACAAAAAACAACCCTTCTTGTAGAACCATGTCGTCAG4890.12011702198215193No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC4710.1156955365104163No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC4250.10439618474931404No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA4170.1024310800952093No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC4170.1024310800952093No Hit
AAAACCCCACTCTATCTTACAACCTAAATATCCCCTGTAGAACCAAAAAA4130.10144852776815694No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)