FastQCFastQC Report
Mon 5 Sep 2022
EGAF00000888495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446077
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT200304.490256166536271No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA132602.9725809669631027No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT131202.941196250871486No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG114682.570856600990412No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT101772.281444683317006No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA94052.1083803917260924No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC87421.959751343377937No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT86851.9469732803977786No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA86541.940023807548921No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA83991.8828587889534767No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC80431.8030519394633662No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA78181.752612217173268No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC73731.6528536553106303No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG71641.606000757716717No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT71261.5974820490632782No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC71111.5941194009106052No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA69801.5647522737105926No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC68661.5391961477502762No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA67811.5201411415517947No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA61071.3690461512250127No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA58451.3103118968249876No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC58251.3058283659547567No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA54851.229608341160831No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC53811.20629398063563No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA49851.117520069405058No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC44570.9991548544309614No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA43530.9758404939057606No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT38920.8724951073469378No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA38270.8579236320186874No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC35580.7976201418140814No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA30350.6803758095575427No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC29020.6505603292705071No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC28700.6433866798781376No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT27140.6084151390903364No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACGCCAACAT26880.6025865489590362No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA24130.540937999493361No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCGCAAAAC23450.5256939945345759No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA22140.4963268673345633No Hit
CAACTACCAAACCCTAAAAAACCACAAAAATTAAAACGAAACCAAAAACC20710.4642696216124122No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT20690.4638212685253891No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCA19900.446111321587977No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT19790.4436453796093499No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAGCGACCATCAAACA19330.4333332586078188No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACACCAAACCCCCACAAAAC19310.4328849055207957No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT18040.4044144844948294No Hit
AAACTACAAAATCAAAACCAACCCAAAACCGCACCAAACCCCCACAAAAC17490.39208477460169433No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA16960.38020341779558237No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA16630.37280559185970136No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA15750.35307805603068526No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC14490.3248318115482305No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC13470.30196580411005275No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC12520.28066903247645586No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC12040.2699085583879016No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA11020.2470425509497239No Hit
AAACTACAAAATCAAAACCAACCCGAAACCGCACCAAACCCCCGCAAAAC10930.24502496205812No Hit
TAAAACTAAACCCCACATACCTTCCCTCTACTCAACAAAGAACTAAAAAC10200.22866007438177716No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT10100.22641830894666168No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC9440.21162265707489963No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT9050.2028797718779493No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC8460.1896533558107681No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC8390.18808412000618727No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT8260.18516982494053716No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAAGAT7930.17777199900465615No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA7310.16387305330694027No Hit
AAAACAAAAAAAACTCCTAATCCCTCCTACGAATTTCTATATTCAAATAT7280.16320052367640564No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAACCAATACC7060.15826863971915162No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA6060.135850985367997No Hit
TAAAACTAAACCCCACATACCTTCCTCCAGATATAAAACCACCAAACGCT5400.12105533349623496No Hit
ATAATACTCCTTTATCCCAATCCCCCACCAAAACTCAACAACCTAAACCC5300.11881356806111949No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACCACACC5250.11769268534356175No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC4940.11074321249470384No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA4900.10984650632065765No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT4880.10939815323363455No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC4500.10087944458019579No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)