FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217824
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT101684.66798883502277No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA70613.2416079036286174No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC55212.5346151021007786No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT54852.5180879976494785No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC51592.3684258851182607No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA46892.1526553547818423No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT46852.150819009842809No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA45092.0700198325253414No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC42311.9423938592625238No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG37761.7335096224474804No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA37691.730296018804172No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA34651.5907338034376377No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT32681.5002938151902452No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA29991.3767996180402526No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA29181.3396136330248274No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC28521.309313941530777No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT27921.281768767445277No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA27851.2785551638019685No Hit
CTCCCAACCTAACCTAAAACCTACTACTACTCCAACCATCTCCATAACAA26291.206937711179668No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT25411.1665381225209344No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA25071.1509291905391508No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC24961.1458792419568091No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT24831.1399111209049508No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG24311.1160386366975172No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA23861.0953797561333922No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG23321.070589099456442No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA23051.0581937711179668No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA22931.0526847363008667No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA21690.9957580431908329No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT21210.9737219039224329No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA21180.9723446452181577No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT19200.881445570736007No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT18870.866295724988982No Hit
CTCCCAACCTAACCTAAAACCTACTACTACTCCAACCATATCCATAACAA18730.8598685177023653No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA18200.8355369472601734No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC17330.7955964448361981No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC17050.7827420302629645No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA16110.7395879241956809No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA15390.7065337152930806No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC15370.705615542823564No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA13540.6216027618627883No Hit
TAAAACTCACTACTATCCCCCACAAACTAGAACTAAAAAACAAACTCTAA12030.5522807404142794No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA10510.48249963273101215No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA9810.45036359629792866No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT9430.4329183193771118No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGGAACCCCGAAAAA9290.42649111209049506No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT9210.4228184222124284No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA9000.41317761128250335No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA8980.4122594388129866No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT8750.4017004554135449No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCGAAA8250.37874614367562803No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCGAA8130.37323710885852796No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC7770.35671000440722783No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT7690.35303731452916115No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA7440.34156015866020273No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC7210.331001175260761No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC7170.3291648303217276No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT7060.3241148817393859No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT6710.3080468635228441No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA5880.26994270603790216No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT5640.2589246364037021No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC5430.24928382547377698No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA5350.24561113559571032No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA5020.2304612898486852No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA4880.22403408256206847No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA4860.22311591009255177No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG4730.21714778904069343No Hit
AAATTACAAAAATAAACTAAAATCCCAACGCATCGAAAATACAATAATAC4170.19143895989422655No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA3860.17720728661671808No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACGACCCAAACACTA3850.17674820038195976No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCGAACACTA3770.17307551050389305No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACACCTACTCCA3620.166189216982518No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCAAAA3320.1524166299397679No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA3230.14828485382694284No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCAAATATCTA3170.14553033641839283No Hit
ATATCTCCTTACATCCCAACCTCGTCTATACAACAAAAAACCAAAAACTT3090.14185764654032615No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA3020.1386440428970178No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTGCTAACCT2970.1363486117232261No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT2950.13543043925370943No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA2930.13451226678419273No Hit
ATATAATACCCACCTTCCAAACCACGAACCCACAACCCAATCCTAAAAAC2710.12441236961950933No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA2610.11982150727192595No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACTCAAATATCCT2400.11018069634200088No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT2330.10696709269869252No Hit
CCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC2300.1055898339944175No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)