FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203415
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT95784.7086006440036385No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA65153.2028119853501464No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA56872.7957623577415625No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC53032.6069857188506256No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC47282.3243123663446648No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA45152.219600324459848No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC44842.2043605437160485No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT43062.1168547058968117No Hit
CTCCCAACCTAACCTAAAACCTACTACTACTCCAACCATATCCATAACAA41772.0534375537693874No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA39421.9379101836147776No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT36831.810584273529484No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA35231.73192734065826No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA34981.7196371948971314No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAATATCTAAAA33501.6468795319912495No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT31311.5392178551237619No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG31031.5254528918712975No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC30941.5210284393972913No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT28371.3946857409728879No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT27221.3381510704716957No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG26571.306196691492761No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA26351.2953813632229678No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA25391.2481872035002335No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC25061.2319642110955435No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA22641.1129956001278176No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC22601.1110291768060367No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT21211.042695966374161No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT20931.028931003121697No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA20921.028439397291252No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA20361.0009094707863235No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA20070.9866529017034142No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA17670.8686675023965784No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT17170.8440872108743209No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA16240.7983678686429222No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC15870.7801784529164516No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT15680.7708379421379937No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA15630.768379912985768No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA14780.7265934173979303No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA14540.7147948774672468No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA13850.6808740751665315No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCGAAA13420.6597350244573901No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG11880.5840277265688371No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT11510.5658383108423666No Hit
CCCTTTAAACTATCCCACCTTTCTAAACAAAACCAATATACATCTTAAAT10840.5329007202025415No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAGACATATCCTAAACCA9830.4832485313275815No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC9730.47833247302313003No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCGAAA8480.41688174421748647No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC8480.41688174421748647No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT8280.40704962760858343No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA7590.3731288253078681No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA6980.34314086965071405No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC6980.34314086965071405No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT6750.33183393555047563No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA6410.3151193373153406No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT6050.29742152741931516No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG5620.27628247671017375No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC5590.2748076592188383No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCAAA5520.2713664184057223No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCAAATATCTA5510.2708748125752771No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA5110.25121057935747115No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAGAAATA4950.2433448860703488No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA4240.20844087210874324No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT4140.20352481380429172No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA3750.1843521864169309No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTGAAACCCCCTCCTTCC3660.17992773394292458No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA3370.16567116486001524No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT3290.16173831821645404No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCA3020.14846496079443502No Hit
CCAAAACTCAACAACCTAAACCCCAAAAATAACTACTAAACTACCTACCG2880.14158247916820293No Hit
ACCAAACAACCAAACTAAACAACCAAACTAAAATCTACTCTTCCCTCCCC2870.14109087333775777No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACGACCTAAATCC2840.13961605584642234No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAACTA2670.1312587567288548No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCGATCCCCTTCCTCCCAT2560.12585109259395819No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT2550.12535948676351302No Hit
ATATAATACCCACCTTCCAAACCACGAACCCACAACCCAATCCTAAAAAC2490.12240985178084213No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCGATCCCCTTCCTCCCAT2460.12093503428950668No Hit
CCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT2400.11798539930683578No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT2320.1140525526632746No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA2320.1140525526632746No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2220.10913649435882311No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2200.1081532826979328No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA2190.10766167686748765No Hit
CACAAAACACCTAAATTATACCCAAAGGAACTAGTCCCGCCGGGTGTCTA2190.10766167686748765No Hit
CCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC2090.10274561856303616No Hit
TCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA2080.10225401273259102No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA2060.10127080107170071No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)