FastQCFastQC Report
Sat 3 Sep 2022
EGAF00000888502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274517
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGAGGGAAGAGTAGATTTTACGATATTTTTGGGGTTTTTAGATAGTT154925.643366348896425No Hit
GGTAGGAGGGGTTGTATAAGGTTTATTTGGAAGTTAGAATTTATTTGTGG110964.042008327353133No Hit
GGAATTGTTTTTGGGAATTTGTAGCGTTGGTTTCGTTAAGTCGGTTGGAA79262.8872528841565366No Hit
GGGGATTGGGATAAAGGAGTATTATTTTCGGTTTTTGTTTGAGTGATTTC73512.6777940892549457No Hit
TAGAAAGGTGGGATAGTTTAAAGGGGGATTTTTAGGTTATAGGTAAATTT71892.618781350517454No Hit
AAAGAGATTTATTTTGAGTGATTATGGTTCGTGATATAGTTTTAGGAGAT66862.435550439499193No Hit
GTTTAGGATTGGATTGAGGATTAAATTTTTTAAGGTATTTTTTTAAAGAG66632.4271720877031293No Hit
GTTGGGATTTTAGTTTATTTTTGTAATTTAAATATTTTTTAGTAAAGTTT60902.2184418451316312No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTTAGGGTTTCGTTTTCGTTTAG59602.171085943675619No Hit
GGATTATTAGGGAAGGGTTTTTTTTCGTTAGCGTTATAAGGGTATTTGAA56872.0716385506179944No Hit
GAAGTTGTGGAATTTAGGTAGGAAGTGGTTGATTTTTAGTGTTTAGTTGT54281.977291023871017No Hit
TGTGTGTGTGAATTTAAAAGTGTGATAATAAATATTATTTTTATTAGTTG51581.878936459308531No Hit
GGTTGGAGTTTTAGGTGTATTTGGGCGTAGGTTTTTTTGAGGAAATTAAG46121.6800416731932812No Hit
TTGGTGGGGAGGTTTTGATAGGTTTTGGGTTTGGGTTTAGGACGGTGAGG45091.6425212281935182No Hit
GGTAGTTGGGTGATTTTTTTATAGGAGTTTAGGTGGTCGTTGACGGTTTT42591.5514521869319569No Hit
TTGTGGGGATTTAGGTTGTATTATGCGGATGAGGTTTTTAGGTAGTAGGT42331.5419810066407544No Hit
GTGTTTGGAGTGTTGTTTTTTTAGGGATGGTGTTTGTAGTGATAGGGTTG41681.5183030559127486No Hit
GTAAGGTTTGGGGTTATTTTAGTGGAGTCGGAGGTGTTACGTTTTGTTGT41091.4968107621750202No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTATT40891.4895252388740952No Hit
GGTTAGGAGAATTTTAGGATTTTGGGTTTTTTTTATAGTAGATTGGAGGA37801.3769639038748056No Hit
AAAATATTAGAGGAAGGAGGGGTTTGGGTTTTGTTGGTTTTGTTTAAAAA36211.3190439936324527No Hit
GGGAGGTAAGAGAGTGTGGATAGTATAGAAATAGTGTGGGGTTGTGGTGG36091.3146726796518977No Hit
TTGTGGGGATTTAGGTTGTATTATGTGGATGAGGTTTTTAGGTAGTAGGT35701.300465909215094No Hit
GTTTGGAGGAGTTGGTTTTTTAGAGTTGGTGTTGTTGGTGTTGTATTTTG34951.2731451968366259No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTA34911.2716880921764409No Hit
AAAGATAATAAATTGGGTATTGTTGAAATGTAATTAATTAAATAGTTTTT32731.1922758881963595No Hit
GTTTATTGTTTGTTGTGTGTGTGGTGTGTTTATATGTGGTTGGGTATTTG32341.178069117759556No Hit
GTAAGGTTTGGGGTTATTTTAGTGGAGTTGGAGGTGTTACGTTTTGTTGT31791.1580339286820123No Hit
TTGAGGTTATTAGAAGGGAGGTTTTAGTTATATAGGGTGGAAGAATTTAA31381.1430986059151165No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTCGGTTTGTTTTTAATTTTTTAG30261.102299675429937No Hit
GAGAGTAGTAGGAGGAGGAGGAGGAGGGGGTAAGTGAGAGGGGGTGTGGT29251.0655077827602661No Hit
GGATAAGAGATAGGTTTGTGTTTTGAATTTGGTTTTTTTTGTTTGTTTGT29061.0585865356243875No Hit
GGAGGAGGGTTTAGTAGGTGGCGAGGGGAAGGAGGGGGTTTTAGGGGGCG22790.830185380140392No Hit
GGAGGAGGGTTTAGTAGGTGGTGAGGGGAAGGAGGGGGTTTTAGGGGGTG22150.8068717055774324No Hit
GATATTTGAAAGGAGGATTGAATGATATGAGGGAGTGGGTCGGAAGGGAG19010.6924889897529114No Hit
GGAAGTTAGGTTTTTAGGAGTGTGGTTGGGTGGGGAGTAGGAGAAGGGTT17770.6473187452871771No Hit
TAGATGTAATAGGTTTTTTTGGGGGGATTTTTAGGGAGTGGTTGGGTAGA17150.6247336230543099No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTCTGGTTTTTTTTTATT16060.5850275210642693No Hit
TTTGGTTGGTTTTTTGTGTTTGTGTTATGTGTTACGGAGGAGAGGGGTTT14440.5260147823267776No Hit
AAAATATTAGAGGAAGGAGGGGTTTGGGTTTCGTTGGTTTTGTTTAAAAA14280.5201863636860377No Hit
GATATTTGAAAGGAGGATTGAATGATATGAGGGAGTGGGTTGGAAGGGAG13900.5063438694142804No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTTGGTTTGTTTTTAATTTTTTAG13610.4957798606279392No Hit
AAAGAGATTTATTTTGAGTGATTATGGTTTGTGATATAGTTTTAGGAGAT13410.4884943373270143No Hit
TGTTTGTTTTGGGATTTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGTA13310.48485157567655196No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTCGGTAGGGTTGAGCGTTAGGTT13210.4812088140260895No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTATGAATTTTTTTTTTAT12510.45570948247285237No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGTG11780.4291173224244765No Hit
GGAAGTTAGGTTTTTAGGAGTGTGGTCGGGTGGGGAGTAGGAGAAGGGTT11070.40325371470619303No Hit
GGTTGGAGTTTTAGGTGTATTTGGGCGCAGGTTTTTTTGAGGAAATTAAG10510.38285424946360336No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTTGGGTGTAGGGGATAA10240.37301879300735474No Hit
GTTGTGGTTATTGGTTTTGTGTCGTTTTTTTTTTTGGGCGTAGGGGATAA10210.371925964512216No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGCG10170.37046885985203104No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTACGAATTTTTTTTTTAT8990.3274842723765741No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTCGTGGTG8510.30999901645435435No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTTGTGGCG7910.2881424465515797No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTTGGTAGGGTTGAGTGTTAGGTT7690.2801283709205623No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTTGTTTTTTTTTA7190.26191456266825003No Hit
GGATAAGAGATAGGTTTGTGTTTTGAATTCGGTTTTTTTTGTTTGTTTGT6600.2404222689305216No Hit
GGAGGAGGGTTTAGTAGGTGGTGAGGGGAAGGAGGGGGTTTTAGGGGGCG6430.23422957412473547No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGCCGGTAGGGTTGAGCGTTAGGTT6200.2258512223286718No Hit
GTTGTGGTTATTGGTTTTGTGTCGTTTTTTTTTTGGGCGTAGGGGATAAG5910.2152872135423307No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTGGGTGTAGGGGATAAG5630.20508748092103588No Hit
TTTGGTTGGTTTTTTGTGTTTGTGTTATGTGTTATGGAGGAGAGGGGTTT5590.20363037626085087No Hit
ATTATTTTTTTAGGGAGTTGGGTTGTATTTTATTTGTTGTTTTGTTTTTA5410.19707340529001846No Hit
GAGAGTAGTAGGAGGAGGAGGAGGGGGTAAGTGAGAGGGGGTGTGGTAGG5240.1908807104842323No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTAGGGTTTCGTTTTCGTTTAGT5060.1843237395133999No Hit
AAAGATAATAAATTGGGTATTGTTGAAACGTAATTAATTAAATAGTTTTT4830.17594538771733625No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTT4830.17594538771733625No Hit
GGTAGTTGGGTGATTTTTTTATAGGAGTTTAGGTGGTTGTTGACGGTTTT4800.17485255922219753No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTATTT4260.15518164630970033No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTAG4180.15226743698933037No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTCGGGCGTAGGGGATAA4170.1519031608242841No Hit
GGAAGTTAGGTTTTTAGGAGTGTGGTTGGGTGGGGAGTGGGAGAAGGGTT3780.13769639038748058No Hit
GGAAGTTAGGTTTTTAGGAGTGTGGCGTTTGAGTTGAGATTTTAGTGAAA3760.13696783805738805No Hit
GGTTAGGAGAATTTTAGGATTTTGGGTTTTTTTATAGTAGATTGGAGGAG3630.13223224791178687No Hit
GGTAGTTGGGTGATTTTTTTATAGGAGTTTAGGTGGTTGTTGATGGTTTT3550.1293180385914169No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGCCGGTAGGGCTGAGTGTTAGGTT3500.12749665776618568No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTCGGTAGGGTTGAGTGTTAGGTT3360.12239679145553826No Hit
GGGGATTGGGATAAAGGAGTATTATTTTCGGTTTTTGTTTGAGTGATTTT3210.1169326489798446No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTATGAATTTTTTTTTATT3000.10928284951387346No Hit
GAAGTTGTGGAATTTAGGTAGGAGTGGGGGTTGGGAGAGACCAAGTCTCT2800.10199732621294856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)