FastQCFastQC Report
Sat 3 Sep 2022
EGAF00000888503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274517
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA92813.3808470877941983No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT85633.1192968012909947No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT78402.8559251339625598No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC75642.7553849124097964No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA73482.676701260759807No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA69302.524433823770477No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC59322.1608862110543243No Hit
CTCCCAACCTAACCTAAAACCTACTACTACTCCAACCATATCCATAACAA58222.1208158328992375No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC56572.060710265666607No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA52891.9266566369295892No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA52881.9262923607645428No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT52781.9226495991140804No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA51811.8873148111045945No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA47421.727397574649293No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG44761.6305001147469922No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT43781.59480105057246No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT43651.5900654604268587No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA37151.3532859531467996No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG35191.2818878247977357No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC34421.2538385600891748No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC33851.2330748186815388No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA32201.1729692514489085No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA31741.156212547856781No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT29321.06805771591559No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA29121.060772192614665No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA28471.037094241886659No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT28231.0283516139255493No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT28021.020701814459578No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC27981.019244709799393No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA26300.958046314071624No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA26250.9562249332463928No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA25870.9423824389746354No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCAAAA24220.8822768717420051No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT24160.8800912147517276No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA20790.7573301471311431No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA20570.7493160715001257No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT19910.7252738446070734No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG19560.7125241788304549No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT19520.7110670741702699No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC19520.7110670741702699No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA19510.7107027980052237No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA17520.638211841161021No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT17410.6342048033455122No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC15680.5711850267925119No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC15120.5507855615499223No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA15100.5500570092198297No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA14850.5409501050936736No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT12930.47100908140479464No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCAAATATCTA11360.4138177234925342No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA10960.3992466768906844No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC9940.3620905080559674No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCACATAATACAACCTAAATCC9930.3617262318909212No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT9450.3442409759687014No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT8930.3252986153862966No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC8540.3110918449494931No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCAAAACCCATTCGAA7600.2768498854351461No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA7550.27502850460991485No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA7430.27065719062935994No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT6640.2418793735907066No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG5800.211280175726822No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT5620.2047232047559896No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCAA5520.20108044310552714No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA5470.1992590622802959No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT5180.18869505349395485No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC5110.18614512033863112No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA5010.18250235868816866No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA4490.16355999810576394No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA4160.15153888465923784No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA3950.1438890851932667No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA3930.14316053286317423No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT3810.1387892188826193No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA3550.1293180385914169No Hit
AACTTCTCCTATCCCCTCCCTACACACGACCACCTACTTTTCACTAAAAT3420.12458244844581574No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT3350.12203251529049203No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTGA3070.11183278266919718No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA3060.11146850650415094No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT3020.11001140184396595No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAACAACTT2850.10381870703817979No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT2810.1023616023779948No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)