FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128444
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC77506.033757902276479No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA76085.923203886518638No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC60654.721902151910561No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT57364.46575939709134No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA55384.311606614555759No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT46953.6552894646694276No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC44803.4879013422191774No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA44413.4575379153561085No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA42963.3446482513780325No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCGAAA40373.143003955030986No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA38482.9958581171561147No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC36662.8541621251284606No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT35092.7319298682694404No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT34712.702344990813117No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG28772.239886643206378No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA28312.2040733704960918No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA25311.9705085484724862No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTGAAACCCCCTCCTTCC20811.6201613154370778No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA17971.3990532839213976No Hit
CCCTCCTTCCTCTAATATTTTACAACAAACAATAAACTGTAGAACCATGT14421.1226682445267977No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA14201.1055401575784No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC11810.9194668493662609No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA10130.7886705490330416No Hit
ACCAAACAACCAAACTAAACAACCAAACTAAAATCTACTCTTCCCTCCCC8790.6843449285291644No Hit
ACCAAACAACCAAACTAAACAACCAAACTAAACAACCAAACTAAACAACC8300.6461960075986423No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA4410.34334028837470026No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA4260.33166204727351994No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT4110.3199838061723397No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA4030.3137554109183769No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA4010.3121983121048862No Hit
ACCAAACAACCAAACTAAACAACCAAACTAAACTAAAATCTACTCTTCCC3290.25614275481922083No Hit
CCTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT2700.21020833982124507No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT2650.2063155927875183No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2580.20086574694030082No Hit
ACCAAACAACCAAACTAAACAACCAACTAAACAACCAACTAAACAACCAA2520.19619445049982873No Hit
CAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC2400.18685185761888448No Hit
CCTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA2150.1673881224502507No Hit
CACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCGAAA2140.16660957304350535No Hit
AATCTAAAAACCTCCTCTCCAAAACCTTATACAACCCCTCCTACCTGTAG2130.16583102363675997No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2070.16115972719628788No Hit
CAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC2040.1588240789760518No Hit
CCTCAAACTAATACACCACTACATACCGCCCCCTGAAACCCCCTCCTTCC1800.14013889321416337No Hit
CACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT1750.13624614618043662No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCAA1710.1331319485534552No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCAAC1540.11989660863878421No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)