FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151860
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT55123.6296588963519034No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA49043.2292901356512576No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC35372.329118925325958No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT34592.277755827736073No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC31472.072303437376531No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC31082.0466218885815883No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA30061.9794547609640458No Hit
CTCCCAACCTAACCTAAAACCTACTACTACTCCAACCATATCCATAACAA29181.9215066508626366No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC28281.8622415382589228No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT25611.686421704201238No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA25311.6666666666666667No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA25201.6594231529039907No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA25081.651521137890162No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA23881.5725009877518767No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC23181.5264059001712103No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT22601.4882128276043725No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA22081.453970762544449No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAAACCCGCCGAATATCTA21691.428289213749506No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT20981.3815356249176873No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA19811.3044909785328593No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA19741.2998814697747927No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC19091.257078888449888No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT18461.2155933096272882No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG18131.1938627683392598No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA16841.108916106940603No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT16521.087844066903727No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG16371.0779665481364415No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA15861.0443829843276702No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA15521.0219939417884893No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA15261.0048729092585276No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA14620.9627288291847754No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA13190.8685631502699855No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT13070.8606611352561571No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCGAAA12400.8165415514289478No Hit
CCCTTTAAACTATCCCACCTTTCTAAACAAAACCAATATACATCTTAAAT11950.7869089951270907No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT11570.7618859475833004No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT11390.7500329250625576No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC10180.6703542736731201No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAAACCCACCGAATATCTA9540.6282101935993678No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA9420.6203081785855393No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA8690.5722375872514158No Hit
ACCAAACAACCAAACTAAACAACCAAACTAAAATCTACTCTTCCCTCCCC8570.5643355722375872No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC8000.5268010009219017No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA7780.5123139733965495No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCGATCCCCTTCCTCCCAT7780.5123139733965495No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG7230.4760964045831687No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCAAAAAA6950.45765836955090217No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT6650.4379033320163308No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC6580.4332938232582642No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT6270.4128802844725405No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC6190.4076122744633215No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCGAACACTA6120.4030027657052548No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT5620.370077703147636No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA5560.3661266956407217No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCATAAAATTC5500.36217568813380746No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT5270.3470301593573028No Hit
CCAAAACTCAACAACCTAAACCCCAAAAATAACTACTAAACTACCTACCG5030.33122612932964574No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCAAAA4870.32069010931120767No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC4850.3193731068089029No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA4810.31673910180429343No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCAAACACTA4660.3068615830370078No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT4580.3015935730277888No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA4090.2693270117213223No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA3910.25747398920057946No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC3880.25549848544712234No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA3330.2192809166337416No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT2970.195574871592256No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG2930.1929408665876465No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA2520.16594231529039907No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA2500.16462531278809428No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT2430.16001580403002766No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAAACCCACCAAATATCTA2370.1560647965231134No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA2370.1560647965231134No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA2350.15474779402080863No Hit
TCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA2310.15211378901619912No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATCACCTTCCAAAAACTCT2310.15211378901619912No Hit
AATCTAAAACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2280.150138285262742No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCACCTCCGA2210.14552877650467536No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCGAATATCTA2200.14487027525352297No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT2170.14289477150006585No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT2140.14091926774660873No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2020.1330172527327802No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACGACCCAAACACTA1970.12972474647701832No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT1880.1237982352166469No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAAACCCGCCAAATATCTA1790.11787172395627551No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA1770.11655472145397076No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA1700.11194521269590413No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA1670.109969708942447No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA1640.10799420518898986No Hit
CCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC1570.10338469643092323No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA1520.10009219017516134No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)