FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218065
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT75413.4581432141792585No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA65603.008277348497008No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT59102.710201086831908No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA52412.403411826748905No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT51012.3392107857748834No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC50542.317657579162176No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA43271.9842707449613646No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC41451.900809391695137No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA41191.8888863412285326No Hit
CTCCCAACCTAACCTAAAACCTACTACTACTCCAACCATATCCATAACAA40011.8347740352647146No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC37091.7008690069474697No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC36301.6646412766835577No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG36231.6614312246348566No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG35611.632999335060647No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA35401.623369178914544No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA35071.6082360763992387No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT34801.5958544470685347No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA34671.5898929218352325No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT31771.456905051246188No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA31301.4353518446334808No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC30421.3949969045926673No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT27131.2441244583037168No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA27011.238621511934515No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA26721.2253227248756104No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA26621.220736936234609No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT26481.2143168321372066No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT26461.2133996744090065No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC26111.1973494141655012No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG25241.1574530529887879No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT24131.1065507990736707No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA24091.10471648361727No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC22701.0409740215073486No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCACATAATACAACCTAAATCC20670.9478825120950175No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA19770.9066104143260038No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA18830.8635040011005892No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT16450.7543622314447527No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA16010.734184761424346No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA15840.7263889207346433No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAATATCTAAAA15600.7153830279962397No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT14940.6851168229656295No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA14170.6498062504299177No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA13750.6305459381377112No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA13490.6186228876711072No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC12410.5690963703482906No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT12330.5654277394354894No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA9600.4402357095361475No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA9560.4384013940797469No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT9450.4333570265746452No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCAAAA7920.36319446036732167No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA7380.33843120170591334No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC7340.33659688624951273No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC6970.3196294682778071No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACGACCCAAACACTA6470.29670052507279937No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA6440.2953247884804989No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT6080.2788159493728934No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG5750.2636828468575883No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT5300.24304679797308143No Hit
ACCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA4820.22103501249627405No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA4770.21874211817577327No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAACAACTT4570.2095705408937702No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT4510.20681906770916927No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACGCCCCCAAAATTA4480.2054433311168688No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC4300.19718891156306606No Hit
CCTAAAACAAACTAATACCACCCACTAAACAAAAACGAAACCCTAAAAAA3870.17747002040675947No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAGAAACTCCCCACAAAAACAAA3730.1710499163093573No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA3550.16279549675555452No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCC3510.16096118129915393No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT3510.16096118129915393No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT3460.15866828697865315No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA3380.15499965606585192No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT3350.15362391947355147No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA3240.14857955196844977No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA2950.1352807649095453No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA2920.13390502831724488No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA2780.1274849242198427No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA2720.12473345103524178No Hit
ATATCTCCTTACATCCCAACCTCGTCTATACAACAAAAAACCAAAAACTT2420.11097608511223718No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2240.1027216655584344No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)