FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173115
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT81224.691678941743927No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA59303.4254686191260144No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT48402.795829361984808No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC44412.5653467348294487No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA43862.533575946625076No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA41392.3908962250527104No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA37242.151171186783352No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA36472.1066920832972302No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT35622.0575917742541088No Hit
CTCCCAACCTAACCTAAAACCTACTACTACTCCAACCATATCCATAACAA34231.977298327701239No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC34201.9755653756173643No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC33341.9258874158796175No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA31281.8068913727868758No Hit
CCTAAAACAAACTAATACCACCCACTAAACAAAAACGAAACCCTAAAAAA30211.745082748462005No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT30021.7341073852641307No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG27301.5769863963261417No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA25881.4949599976893972No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC23581.3621003379256562No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA23521.3586344337579066No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC22791.3164659330502844No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT22571.3037576177685353No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT22401.293937555959911No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT22341.2904716517921613No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT21861.2627444184501633No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT21611.2483031510845393No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA21231.2263524246887907No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG20911.207867602460792No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC20841.2038240475984172No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA19561.1298847586864222No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA18611.075007942697051No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT17561.0143546197614304No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA17090.9872050371140572No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA16370.94561418710106No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA15350.886693816249314No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC14160.8179533835889439No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC13310.7688530745458221No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCACATAATACAACCTAAATCC13150.7596106634318228No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG12830.7411258412038241No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA12410.7168645120295757No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC10610.6128873869970829No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA8610.4973572480720908No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC8110.4684747133408428No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT7760.4482569390289692No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA7530.4349709730525951No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT7340.42399560985472085No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC7110.41070964387834674No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA6390.3691187938653496No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA5920.34196921121797647No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG5880.33965860843947665No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT5560.32117378621147796No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT5240.3026889639834792No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCGATCCCCTTCCTCCCAT5210.3009560118996043No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA4970.28709239522860525No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA4730.2732287785576062No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA4410.2547439563296075No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA4400.25416630563498255No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAGATCCTCC4030.23279322993385898No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCA3690.21315310631661033No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA3540.20448834589723597No Hit
ACTACAAACCCTACAACAAATCCATGCCCCCCGCCACAACCCAAACACTA3010.17387285908211306No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT3010.17387285908211306No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA3000.17329520838748808No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA2940.16982930421973833No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT2820.1628974958842388No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA2590.14961152990786472No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAATTTC2520.14556797504549No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA2510.14499032435086504No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA2460.14210207087774024No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA2090.1207289951766167No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAGCCAATATACATCTTAAAT1980.11437483753574214No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA1900.10975363197874245No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT1850.10686537850561764No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA1810.1045547757271178No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)