FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194022
Sequences flagged as poor quality0
Sequence length151
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT133386.874478151962149No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT101815.247343084804816No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC75383.885126428961664No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA72483.73565884281164No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA70443.63051612703714No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA68923.552174495675748No Hit
CTCCCAACCTAACCTAAAACCTACTACTACTCCAACCATATCCATAACAA68243.517126923750915No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC57012.9383265815216832No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA55102.8398841368504604No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC49792.566203832555071No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA47082.426528950325221No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA43912.26314541649916No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA38051.9611178113822143No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAACAACTT32901.6956839945985507No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT32801.6905299399037221No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT30321.5627093834719774No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA25191.2983063776272794No Hit
ATATCTCCTTACATCCCAACCTCGTCTATACAACAAAAAACCAAAAACTT24901.283359619012277No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT23321.2019255548339878No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT23041.1874942016884682No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT22231.145746358660358No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT21871.1271917617589757No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC20741.0689509437074145No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCGATCCCCTTCCTCCCAT19280.9937017451629196No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG16130.8313490222758244No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT15360.7916628011256456No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACGACCTAAATCC15000.7731082042242632No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA14440.744245497933224No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT12720.6555957571821752No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT12610.649926297017864No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT12420.64013359309769No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT12350.6365257548113101No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT11830.6097246703982022No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC11630.5994165610085455No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG9620.49582006164249415No Hit
AAAAACTAACACACACTTATACCCCCAAAACAACCATAAATACACAAAAA9390.4839657358443888No Hit
CTCCCAACCTAACCTAAAACCTACTACTCCAACCATATCCATAACAACCA8120.4185092412200679No Hit
AAATTACAAAAATAAACTAAAATCCCAACGCATCAAAAATACAATAATAC7110.36645328880230077No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA7080.3649070723938522No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA6190.3190359856098793No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT6180.31852058014039647No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA4140.21337786436589667No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA4070.20977002607951678No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA4050.20873921514055105No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA3760.19379245652554863No Hit
CCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC3220.16596056117347519No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTCCTCCATCTT3100.15977569553968105No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAATAAAAAAAAAAAAA2950.15204461349743842No Hit
CAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA2910.14998299161950707No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT2750.1417365041077816No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT2700.13915947676036738No Hit
CAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC2660.13709785488243603No Hit
ACCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA2520.12988217830967622No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA2440.12575893455381348No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACTAAAAACCCAAATATCCT2370.12215109626743359No Hit
CAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA2160.11132758140829391No Hit
CCTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC1990.1025656884270856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)