FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161055
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT77224.79463537300922No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA70944.404706466734966No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC54513.3845580702244575No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA53403.3156375151345814No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT48673.0219490236254694No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA48373.0033218465741514No Hit
CTCCCAACCTAACCTAAAACCTACTACTACTCCAACCATATCCATAACAA45582.8300890999968953No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA41462.5742758684921303No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT40312.5028716897954117No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC35692.2160131632051163No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT35602.210425010089721No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA35512.2048368569743255No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT34092.1166682189314208No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA31931.982552544161932No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG30471.8919002825121853No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG29661.841606904473627No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT28611.7764117847940144No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC28361.7608891372512494No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA27471.7056285119990064No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA25901.6081462854304431No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT25671.5938654496910991No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA25481.5820682375585982No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT25041.5547483778833318No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA23581.4640961162335848No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA21411.3293595355623855No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA21391.3281177237589643No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA20511.273478004408432No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAAGATATCCTAAACCA19201.192139331284344No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT19101.1859302722672378No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA15240.9462605942069479No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA14320.8891372512495731No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC11920.7401198348390301No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA11910.7394989289373196No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA8490.5271491105522959No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC7020.43587594300083826No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG5800.36012542299214556No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT5690.353295458073329No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT5280.3278383161031946No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA5150.3197665393809568No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT3610.22414703051752508No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT3340.20738257117133896No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT3130.19434354723541647No Hit
AATCTAAAACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA3020.18751358231659992No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA2940.18254633510291515No Hit
CACAAAACACCTAAATTATACCCAAAAAAAACTAATCCCGCCGAATATCT2920.18130452329949395No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA2820.17509546428238798No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA2770.171990934773835No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT2610.1620564403464655No Hit
ACCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA2590.1608146285430443No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2510.15584738132935955No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA2370.14715469870541117No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2260.14032473378659463No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA2220.13784111017975226No Hit
CCTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT2030.12604389804725094No Hit
CCAAAACTCAACAACCTAAACCCGAAAATAACTACTAAACTACCTACCGC1960.12169755673527678No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA1930.119834839030145No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAATAAAACATCTAAAA1840.11424668591474962No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA1750.10865853279935427No Hit
ACCCCACTCCAACTATCCTAAAAAAACAACACTCCAAACACTGTAGAACC1720.10679581509422248No Hit
AACTTCTCCTATCCCCTCCCTACACTTCCTCCCAAATACATTCAAATTCC1620.10058675607711651No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)