FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222495
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT93644.2086339018854355No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA68793.0917548708959752No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT65342.936695206633857No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA57002.561855322591519No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC48552.1820715072248817No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC48072.1604979887188476No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA47042.1142048135913165No Hit
CTCCCAACCTAACCTAAAACCTACTACTACTCCAACCATATCCATAACAA45352.038248050517989No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC42651.916897008921549No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA40711.8297040382929952No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA39631.781163621654419No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC39361.769028517494775No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA38681.7384660329445607No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT34631.5564394705499No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG33881.522730847884222No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA33211.4926178116362165No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT29361.3195802152857368No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG27931.2553091080698444No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC27861.252162969954381No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC26851.206768691431268No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAATATCTAAAA26341.1838468280186072No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT26231.1789028966943078No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA25851.1618238612103644No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA25711.1555315849794376No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA24991.123171307220387No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA24731.111485651362952No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT24651.1078900649452796No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT23851.0719342007685566No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC21740.9771006090024495No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG21730.9766511607002405No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA21630.9721566776781501No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT20820.935751365199218No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT20630.9272118474572463No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA17740.7973212881188342No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT17170.771702734892919No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT16680.7496797680846761No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA16520.7424885952493314No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA15130.6800152812422751No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA14420.6481044517854334No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA13800.6202386570484729No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA12970.5829344479651227No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT11850.5325962381177105No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT11660.5240567203757387No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC10660.47911189015483496No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC10360.4656284410885638No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT9710.4364143014449763No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA9670.4346165082361402No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC9590.43102092181846785No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA9550.42922312860963163No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCAATT9040.4063012651969707No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCATCCTAACCT8480.3811321602732646No Hit
ACCAAACAACCAAACTAAACAACACTAAAAACGAAAAACCCAAATATCCT8310.3734915391357109No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCGAACACTA8140.3658509179981573No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT7780.34967077911863187No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCAAAA7700.34607519270095954No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC7400.3325917436346884No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT6550.29438863794692016No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC6420.2885458100182027No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG6110.27461291264972243No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA5930.2665228432099598No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA5650.2539382907481067No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA5560.24989325602822535No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCATAAAATTC5520.24809546281938918No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT4660.2094429088294119No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT3970.17843097597698823No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCAAA3890.17483538955931593No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCA3890.17483538955931593No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCAAACACTA3850.17303759635047977No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA3770.16944200993280747No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA3540.1591046989819996No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA3540.1591046989819996No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCA3510.15775635407537247No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA3470.1559585608665363No Hit
AACTAATCTAAACCTCCTAACCTCAAACGACCTATCTACTTCTACCTCCA3460.15550911256432728No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA3440.1546102159599092No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT3410.1532618710532821No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA3290.14786849142677364No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA3260.14652014652014653No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA2990.1343850423605025No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCGAAA2930.13168835254724826No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA2910.13078945594283017No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT2870.12899166273399404No Hit
AATCTAAAACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2870.12899166273399404No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTG2850.12809276612957596No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCGAAA2810.1262949729207398No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCAAATATCTA2710.12180048989864942No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA2630.1182049034809771No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATCC2580.1159576619699319No Hit
CCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC2460.11056428234342344No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACAAAACACCCAAAA2420.1087664891345873No Hit
ATATCTCCTTACATCCCAACCTCACCTATACAACAAAAAACCAAAAACTT2400.10786759253016921No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)