FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210959
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT82533.9121345853933702No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA72793.450433496556203No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC57562.728492266269749No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA56362.671609175242583No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT53832.5516806583269735No Hit
CTCCCAACCTAACCTAAAACCTACTACTACTCCAACCATATCCATAACAA48982.321778165425509No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC48352.291914542636247No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA46112.1857327727188696No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA44692.118421115003389No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA43732.072914642181656No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC43322.053479586080708No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA39071.8520186386928266No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT38021.802245934044056No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA34621.6410771761337513No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG31451.4908110106703198No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC28971.3732526225475092No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA26541.2580643632174973No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAGCAAAACGTCTAAAA26011.2329409980138322No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG25681.2172981479813614No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA25541.210661787361525No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT25511.209239710085846No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC25311.1997591949146518No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT24861.1784280357794643No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC24191.146668309955963No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC24041.1395579235775672No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT23331.105902094719827No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA23151.0973696310657521No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA22871.08409690982608No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG22861.0836228840675204No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACGACCCAAACACTA21621.0248436900061149No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA19690.933356718604089No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA19330.916291791295939No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA18540.8788437563697212No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA17290.8195905365497561No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC16970.804421712275845No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT16190.7674477031081869No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT15970.7570191364198731No Hit
AAATTACAAAAATAAACTAAAATCCCAACGCATCGAAAATACAATAATAC15390.7295256424234093No Hit
ATATAATACCCACCTTCCAAACCACAAACCCGCAACCCAATCCTAAAAAC14420.6835451438431164No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT14350.6802269635331983No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT14090.6679022938106457No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA13550.6423049028484208No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA12450.5901620694068516No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA12220.579259476959978No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT11950.5664607814788656No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACGAAACACCCGAAA9850.46691537218132434No Hit
ATATCTCCTTACATCCCAACCTCGTCTATACAACAAAAAACCAAAAACTT9500.45032447063173414No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT8490.4024478690172024No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCGATCCCCTTCCTCCCAT8050.3815907356405747No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA7560.35836347347114844No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA7560.35836347347114844No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA7320.34698685526571516No Hit
CCAAAAAAACCAACAAAAAAACTAACAAAAAAACTAACAAAAAACCACTC7170.3398764688873193No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG5660.26829857934480156No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT5210.24696742020961418No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC5160.2445972914168156No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT4910.23274664745282259No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA4730.22421418379874763No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT4020.19055835494100748No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA3710.17586355642565618No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA3480.1649609639787826No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT3190.15121421698055074No Hit
CTCCCAACCCCCACTCCTACCTAAATTCCACAACTTCTGTAGAACCATGT3160.14979213970487157No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2690.12751292905256473No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA2600.12324669722552724No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT2440.11566228508857171No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA2220.10523371840025787No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)