FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151483
Sequences flagged as poor quality0
Sequence length151
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT93816.192774106665435No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA65964.354283979060357No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA57083.768079586488253No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC55353.6538753523497682No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT51033.368694837044421No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA50773.351531194919562No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA50383.325785731732274No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC44392.930361822778794No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC44312.925080702124991No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA41632.748163160222599No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC37342.4649630651624275No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT33712.2253322154961284No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG33072.1830832502657063No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA31442.0754804169444756No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA28931.9097852564314146No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT28341.8708369916096195No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC28021.8497125089944086No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA27791.8345292871147258No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC27381.8074635437639868No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT27221.7969013024563811No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT22281.4707921020840624No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA20751.3697906695800848No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA20531.3552675877821274No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG19841.3097179221430788No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA18981.2529458751146993No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCACCACAACCCAAACACTA17331.1440227616300178No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA16641.0984730959909694No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCAAATATCTA15601.0298185274915337No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCAAAA11200.7393568915323832No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA9880.652218400744638No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA9830.6489177003360113No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT7560.49906590178435867No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAGAAAAAAAA6710.4429539948377046No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA5740.3789204069103464No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG3850.2541539314642567No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA3820.25217351121908066No Hit
AACCCAATCTAAAACCAAAAACCTAACCGCCGATCCACCTACTAAACCCT3660.2416112699114752No Hit
ACCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA3380.22312734762316563No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2880.19012034353689855No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA2800.1848392228830958No Hit
CCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC2600.17163642124858894No Hit
CCAAAACTCAACAACCTAAACCCGAAAATAACTACTAAACTACCTACCGC2560.16899586092168759No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2420.1597538997775328No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT2340.15447277912373006No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACTAAAAACCCAAATATCCT1920.12674689569126568No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA1910.12608675560954033No Hit
CCTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT1780.1175049345471109No Hit
CAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC1730.11420423413848418No Hit
CTATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT1650.10892311348468145No Hit
CTATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC1630.10760283332123077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)