FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188425
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTAGGAGGGGTTGTATAAGGTTTATTTGGAAGTTAGAATTTATTTGTGG76514.060501525806023No Hit
TGGGGAGGGAAGAGTAGATTTTACGATATTTTTGGGGTTTTTAGATAGTT59083.1354650391402417No Hit
GGGGATTGGGATAAAGGAGTATTATTTTCGGTTTTTGTTTGAGTGATTTC48352.5660076953695103No Hit
GGTTGGAGTTTTAGGTGTATTTGGGCGTAGGTTTTTTTGAGGAAATTAAG43602.3139180045110788No Hit
TAGAAAGGTGGGATAGTTTAAAGGGGGATTTTTAGGTTATAGGTAAATTT42832.27305293883508No Hit
TTGTGGGGATTTAGGTTGTATTATGCGGATGAGGTTTTTAGGTAGTAGGT42202.2396178851001722No Hit
GGATTATTAGGGAAGGGTTTTTTTTCGTTAGCGTTATAAGGGTATTTGAA41912.224227146079342No Hit
TGGGGAGGGAAGAGTAGATTTTATGATATTTTTGGGGTTTTTAGATAGTT40992.1754013533236036No Hit
GTAAGGTTTGGGGTTATTTTAGTGGAGTCGGAGGTGTTACGTTTTGTTGT39972.121268409181372No Hit
GGAATTGTTTTTGGGAATTTGTAGCGTTGGTTTCGTTAAGTCGGTTGGAA38842.0612975985139976No Hit
GTTTAGGATTGGATTGAGGATTAAATTTTTTAAGGTATTTTTTTAAAGAG35601.8893458935916148No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTATT34911.852726549024811No Hit
GTTGGGATTTTAGTTTATTTTTGTAATTTAAATATTTTTTAGTAAAGTTT34901.8521958338861617No Hit
TGTGTGTGTGAATTTAAAAGTGTGATAATAAATATTATTTTTATTAGTTG34851.8495422581929148No Hit
GATATTTGAAAGGAGGATTGAATGATATGAGGGAGTGGGTCGGAAGGGAG34771.8452965370837202No Hit
AAAGATAATAAATTGGGTATTGTTGAAATGTAATTAATTAAATAGTTTTT33741.7906328778028395No Hit
AAAGAGATTTATTTTGAGTGATTATGGTTCGTGATATAGTTTTAGGAGAT31721.6834284197956746No Hit
GTGTTTGGAGTGTTGTTTTTTTAGGGATGGTGTTTGTAGTGATAGGGTTG30001.59214541594799No Hit
GGTAGTTGGGTGATTTTTTTATAGGAGTTTAGGTGGTCGTTGACGGTTTT29241.5518110654106407No Hit
TTGGTGGGGAGGTTTTGATAGGTTTTGGGTTTGGGTTTAGGACGGTGAGG28421.5082924240413957No Hit
TTGAGGTTATTAGAAGGGAGGTTTTAGTTATATAGGGTGGAAGAATTTAA28351.5045774180708504No Hit
GGTTAGGAGAATTTTAGGATTTTGGGTTTTTTTTATAGTAGATTGGAGGA26481.4053336871434259No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTCGGGGTTTCGTTTTCGTTTAG26471.4048029720047766No Hit
GAAGTTGTGGAATTTAGGTAGGAAGTGGTTGATTTTTAGTGTTTAGTTGT25841.3713679182698688No Hit
GGGAGGTAAGAGAGTGTGGATAGTATAGAAATAGTGTGGGGTTGTGGTGG25471.3517314581398434No Hit
GAGAGTAGTAGGAGGAGGAGGAGGAGGGGGTAAGTGAGAGGGGGTGTGGT23471.2455884304099776No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTA23011.2211755340321084No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTCGGTTTGTTTTTAATTTTTTAG22211.1787183229401619No Hit
GTTTATTGTTTGTTGTGTGTGTGGTGTGTTTATATGTGGTTGGGTATTTG22031.169165450444474No Hit
AAAATATTAGAGGAAGGAGGGGTTTGGGTTTCGTTGGTTTTGTTTAAAAA21571.1447525540666046No Hit
GGAAGTTAGGTTTTTAGGAGTGTGGTTGGGTGGGGAGTAGGAGAAGGGTT18921.0041130423245324No Hit
TAGATGTAATAGGTTTTTTTGGGGGGATTTTTAGGGAGTGGTTGGGTAGA18760.9956216001061431No Hit
GGATAAGAGATAGGTTTGTGTTTTGAATTCGGTTTTTTTTGTTTGTTTGT16190.8592278094732653No Hit
GTTTGGAGGAGTTGGTTTTTTAGAGTTGGTGTTGTTGGTGTTGTATTTTG15010.7966034231126443No Hit
AAAATATTAGAGGAAGGAGGGGTTTGGGTTTTGTTGGTTTTGTTTAAAAA13670.7254875945336341No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTTAGGGTTTCGTTTTCGTTTAG13350.7085047100968556No Hit
GGAATTGTTTTTGGGAATTTGTAGCGTTGGTTTTGTTAAGTCGGTTGGAA11870.6299588695767547No Hit
GGATAAGAGATAGGTTTGTGTTTTGAATTTGGTTTTTTTTGTTTGTTTGT11270.5981159612577949No Hit
GGAGGAGGGTTTAGTAGGTGGCGAGGGGAAGGAGGGGGTTTTAGGGGGCG10880.577418070850471No Hit
AAAGATAATAAATTGGGTATTGTTGAAATGTAATTAGTTAAATAGTTTTT10720.5689266286320817No Hit
TTTGGTTGGTTTTTTGTGTTTGTGTTATGTGTTACGGAGGAGAGGGGTTT10580.5614966166909912No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGCG9790.519570120737694No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTTGTGGCG9780.5190394055990447No Hit
TGGGTTTTAGATATTTGTTTTTTTTGGTTGGTTTGTTTTTAATTTTTTAG9700.5147936844898501No Hit
TGTTTGTTTTGGGATTTTTTTTTATCGTTTAGTTTTATTGGGGTTTAGTA9370.4972800849144222No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTACGAATTTTTTTTTTAT9200.4882579275573836No Hit
GATATTTGAAAGGAGGATTGAATGATATGAGGGAGTGGGTTGGAAGGGAG9080.48188934589359167No Hit
GGAGGAGGGTTTAGTAGGTGGTGAGGGGAAGGAGGGGGTTTTAGGGGGTG8800.4670293220114104No Hit
GGTAGTTGGGTGATTTTTTTATAGGAGTTTAGGTGGTTGTTGATGGTTTT8290.43996284994029455No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTCGGTAGGGTTGAGCGTTAGGTT8030.426164256335412No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTATGAATTTTTTTTTTAT7920.4203263898102693No Hit
TGTTTGTTTTGGGATTTTTTTTTATTGTTTAGTTTTATTGGGGTTTAGTA7810.4144885232851267No Hit
GGAGGAGGGTTTAGTAGGTGGCGAGGGGAAGGAGGGGGTTTTAGGGGGTG6680.35451771261775245No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTTGTTTTTTTTTA6540.3470877006766618No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTTGGTAGGGTTGAGCGTTAGGTT6140.3258590951306886No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTTGGGTGTAGGGGATAA6000.318429083189598No Hit
GTTTGGAGGAGTTGGTTTTTTAGAGTTGGCGTTGTTGGTGTTGTATTTTG5740.3046304895847154No Hit
GAGAGTAGTAGGAGGAGGAGGAGGGGGTAAGTGAGAGGGGGTGTGGTAGG5360.2844633143160408No Hit
GTTTGGAGGAGTTGGTTTTTTAGAGTTGGTGTTGTTGGCGTTGTATTTTG5180.2749104418203529No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGCG5170.2743797266817036No Hit
GTAAGGTTTGGGGTTATTTTAGTGGAGTCGGAGGTGTTATGTTTTGTTGT4970.263765423908717No Hit
GGTAGGAGGGGTTGTATAAGGTTTATTCGGAAGTTAGAATTTATTTGTGG4850.25739684224492504No Hit
ATTATTTTTTTAGGGAGTTGGGTTGTATTTTATTTGTTGTTTTGTTTTTA4570.24253681836274382No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGTG4510.23935252753084785No Hit
GTTGTGGTTATTGGTTTTGTGTTGTTTTTTTTTTGGGTGTAGGGGATAAG4340.23033037017380922No Hit
AATGGTTGTTAGAGTTGTAGGGTTTTTTTTAGGGTTTCGTTTTTGTTTAG4310.22873822475786124No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTACGAATTTTTTTTTATT4260.22608464906461456No Hit
GGAGGAGGGTTTAGTAGGTGGTGAGGGGAAGGAGGGGGTTTTAGGGGGCG4260.22608464906461456No Hit
GAAGTTGTGGAATTTAGGTAGGAAGTGGTTGATTTTTGGTGTTTAGTTGT3910.207509619211888No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTATTT3730.19795674671620006No Hit
GTTGTGGTTATTGGTTTTGTGTCGTTTTTTTTTTCGGGTGTAGGGGATAA3700.19636460130025207No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTTT3610.1915881650524081No Hit
GGTAGTTGGGTGATTTTTTTATAGGAGTTTAGGTGGTCGTTGATGGTTTT3560.18893458935916146No Hit
TGTGTGTGTGAATTTAAAAGTGTGATAATAAATATTGTTTTTATTAGTTG3350.17778957144752555No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGCCGGTAGGGTTGAGCGTTAGGTT3050.16186811728804562No Hit
GGAAGTTAGGTTTTTAGGAGTGTGGTCGGGTGGGGAGTAGGAGAAGGGTT2930.15549953562425367No Hit
GGTTGGAGTTTTAGGTGTATTTGGGTGTAGGTTTTTTTGAGGAAATTAAG2900.15390739020830568No Hit
GGGGATTGGGATAAAGGAGTATTATTTTCGGTTTTTGTTTGAGTGATTTT2840.1507230993764097No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTTAT2630.1395780814647738No Hit
GGAAGGTATGTGGGGTTTAGTTTTAAGTTTGGTATCGTCGTTTTTTTTAG2620.13904736632612444No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTTGGTAGGGTTGAGTGTTAGGTT2580.13692450577152715No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTCGGTAGGGTTGAGTGTTAGGTT2500.1326787846623325No Hit
AAAGAGATTTATTTTGAGTGATTATGGTTTGTGATATAGTTTTAGGAGAT2270.12047233647339792No Hit
GGATTATTAGGGAAGGGTTTTTTTTCGCTAGCGTTATAAGGGTATTTGAA2260.11994162133474857No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTCCGGGTTGTGGTG2200.1167573305028526No Hit
GGGTGTTTAGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTTGTGGTG2020.10720445800716466No Hit
GGTTAGGAGAATTTTAGGATTTTGGGTTTTTTTATAGTAGATTGGAGGAG2020.10720445800716466No Hit
GGATTTTTTTGATTTTTTTGTTTTGAATTTTTTATGAATTTTTTTTTATT2010.10667374286851533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)