FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188425
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT77834.130555924107735No Hit
AATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA64113.4024147538808545No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC48092.552209101764628No Hit
CTCCCAACCTAACCTAAAACCTACTACTACTCCAACCATATCCATAACAA43702.319225155897572No Hit
TAAAACTCACTACTATCCCCCACAAACTAAAACTAAAAAACAAACTCTAA42892.276237229666976No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC42252.242271460793419No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT39072.073504046702932No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT36771.9514395648135865No Hit
CTCCAAACTACAAAAAACTCCTTCAAATCACCTATCCACCTCCTAAAACA35781.8988987660873025No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA35541.8861616027597186No Hit
AAAATTTAATCCTCAATCCAATCCTAAACCACCAACATCCTCTATTAATC34771.8452965370837202No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCGCATAATACAACCTAAATCC34021.8054929016850205No Hit
AAAATTAACCTAAAAATCCCCACAACCTTAACTCTACCTATATTCCCACA33841.7959400291893326No Hit
ATATAATACCCACCTTCCAAACCACGAACCCGCAACCCAATCCTAAAAAC32141.7057184556189466No Hit
CCCTTTAAACTATCCCACCTTTCTAAACGAAACCAATATACATCTTAAAT30811.6351333421785856No Hit
AACTAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCG29591.5703860952633673No Hit
ATATCTCCTTACATCCCAACCTCATCTATACAACAAAAAACCAAAAACTT29141.5465039140241474No Hit
AAATAATAACATTAAATACTCCTCCCAACTTAACCAAACTTAAATCCTCC28511.5130688602892397No Hit
CTCCCAACCCCCACTCCTAAACACACCATTCCAAAACATATCCTAAACCA28011.4865331033567732No Hit
CCAAAACTCAACAACCTAAACCCCGAAAATAACTACTAAACTACCTACCG27941.482818097386228No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCG26161.3883508027066471No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA25911.375082924240414No Hit
TCCCTAATCTCAAAAAACATAAACTAACTCTAAAACCAAAAATTTCGATT24701.310866392463845No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA23821.2641634602627039No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCGCCGAATATCTA21891.1617354385033833No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCCGAAAAA21141.1219318031046837No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAACTCTACTAACCCATCTCCGA20701.098580337004113No Hit
CCCAAAATCCTAAAATTCTCCTAACCACAAAAACTCCCCACAAAAACAAA19451.0322409446729468No Hit
CCTAAAACAAACTAATACCACCCACTAAACGAAAACGAAACCCTAAAAAA18770.9961523152447924No Hit
TAATACCAACTATACCACTCAACTCCAAAAAATTACCTTCCAAAAACTCT18440.9786387156693644No Hit
CCCTCCTTCCTCTAATATTTTACAACTATAAAAAAAATAAAAAAAAAAAA18310.9717394188669232No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACATTACTC16490.875149263632745No Hit
AACTTCTCCTATCCCCTCCCTACCCCCATACCTCAAATTTCTCCCTCCAT16320.8661271062757065No Hit
AACCCAATCTAAAACCAAAAACCTACGCATCCAATATAAAAAAAACAACT15130.8029720047764363No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCGAACACTA14680.7790898235372163No Hit
CCACCTAAACTCCTATAAAAAAATCACCCAACTACCGAAACCCATTCGAA14650.7774976781212685No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA14320.7599840785458405No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAAA13020.6909911105214276No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAACTCTACTAACCCATCTCCGAA12410.6586174870638185No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC11170.5928088098713016No Hit
AACCTTTATTTAATCAAAACTACTTAATTCAAAAAATATCCACGTTACTC10760.5710494891866791No Hit
AACCCAATCTAAAACCAAAAACCTACACATCCAATATAAAAAAAACAACT9680.5137322542125514No Hit
CCAACCACTCCCTAAAAATCCCCCCAAAAAAACCTATTACATCTACTCCA9200.4882579275573836No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCGAATATCTA8460.44898500729733315No Hit
CCTAAAACAAACTAATACCACCCACTAAACAAAAACGAAACCCTAAAAAA8090.42934854716730797No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT7890.41873424439432133No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCAATCCCCTTCCTCCCAT7870.4176728141170227No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAACCCCCCCAA7790.4134270930078281No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCAATCCCCTTCCTCCCAT7770.4123656627305293No Hit
ACTAAATAAAAACTTACCCAACCCCGCTCTACCGATCCCCTTCCTCCCAT7700.4086506567599841No Hit
CACAAAACACCTAAATTATACCCAAAAAAACTAATCCCACCAAATATCTA6610.35080270664720714No Hit
TAATACCAACTATACCACTCAACTCCGAAAAATTACCTTCCAAAAACTCT6610.35080270664720714No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAACCCCCCCAAC6520.3460262703993632No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA6070.32214408916014325No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT5820.30887621069391No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC5350.28393259917739155No Hit
CCCAAAATCCTAAAATTCTCCTAACCGCAAAAACTCCCCACAAAAACAAA5210.2765025872363009No Hit
CAAATCCCTAATCTCACCTAAAAAAAAAAAACTCTACTAACCCATCTCCG5060.26854186015656095No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA4870.2584582725222237No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACGACCCAAACACTA4470.23722966697625048No Hit
TACCTTCCAAAAACTCTAAACATCCCCGTACAACTACAAAACACCCAAAA4300.22820750961921188No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACGAAACACCCAAAA3820.20273318296404405No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT3630.1926495953297068No Hit
ACCAAACAACCAAACTAAACAACACTAAAAACAAAAAACCCAAATATCCT3530.18734244394321348No Hit
AATCTAAAACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA3290.17460528061562958No Hit
CAAATCCCTAATCTCACCTAAAAAAAAACTCTACTAACCCATCTCCGAAT3250.17248242006103223No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA3190.16929812922913626No Hit
ACACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACAAAAAATA3180.16876741409048693No Hit
CCACTCTAAAATTTCCCCTAAAAAACTAATTCAAACCATAACGAAAAATA3150.16717526867453894No Hit
CCAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTAACCT3060.16239883242669498No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAACCCCCCCAACA3060.16239883242669498No Hit
CCCTTTAAACTATCCCACCTTTCTAAACAAAACCAATATACATCTTAAAT2960.15709168104020166No Hit
ACAAACCAACCTAAATTAAAATCCCAACTCCTTAATAACCGTCCTGACCT2940.156030250762903No Hit
ACTACAAACCCTACAACAAATCCATACCCCCCGCCACAACCCGAACACTA2890.15337667506965638No Hit
AACTTCTCCTATCCCCTCCCTACCCCCGTACCTCAAATTTCTCCCTCCAT2710.1438238025739684No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCGAAA2590.13745522091017645No Hit
TCTCAAACCTACTACCTAAAAACCTCATCTGCATAATACAACCTAAATCC2420.12843306355313786No Hit
AATCTAAAAATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2390.12684091813718987No Hit
AACAACAATATTTCCTAATCCTCCCAACTAAAAAAAAAAAAACCCCCCCA2320.12312591216664455No Hit
ACTAAATAAAAACTTACCCAACCCCACTCTACCGATCCCCTTCCTCCCAT2220.11781876078015124No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA2100.1114501791163593No Hit
CATCTAAAAACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTA2030.10773517314581398No Hit
TCTCAAACCTACTACCTAAAAACCTCATCCACATAATACAACCTAAATCC2010.10667374286851533No Hit
ATATAATACCCACCTTCCAAACCACGAACCCACAACCCAATCCTAAAAAC1970.104550882313918No Hit
TACCTTCCAAAAACTCTAAACATCCCCATACAACTACAAAACACCCAAGA1940.10295873689797001No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA1930.1024280217593207No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)